PMID: 12594036Feb 21, 2003

Cloning and expression of hyaluronate lyase genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus(1)

FEMS Microbiology Letters
Ayuko Takao

Abstract

Hyaluronate lyase (HAase) genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus were isolated. In S. constellatus subsp. constellatus, the deduced amino acid sequence of HAase was most similar to that of S. intermedius (68%), whereas the enzyme of S. intermedius was most similar to that of S. pneumoniae (72%). Upstream of the HAase gene on the opposite strands, an open reading frame of a putative glutathione peroxidase started in S. intermedius, and this arrangement was similar to that in S. pneumoniae but unlike that in S. constellatus subsp. constellatus. Cell lysates of Escherichia coli carrying each streptococcal gene showed HAase activity, demonstrating that each cloned gene actually coded for HAase.

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Citations

Feb 9, 2012·Infection and Immunity·Carolyn MarionSamantha J King
Dec 17, 2009·Journal of Clinical Microbiology·Margot E GrinwisMichael G Surette
Mar 26, 2009·Nihon saikingaku zasshi. Japanese journal of bacteriology·Hideaki Nagamune
Jul 22, 2005·Microbiology and Immunology·Akiko SukenoHiroki Kourai
Mar 4, 2008·Microbiology·D PecharkiA Aa Scheie
May 23, 2014·Molecular Oral Microbiology·D Asam, B Spellerberg

Related Concepts

Percutaneous Coronary Intervention for ST Elevation Myocardial Infarction-Unstable-Over 12 Hours From Symptom Onset
Alkalescens-Dispar Group
Polysaccharide-Lyases
Sequence Determinations, DNA
Streptococcus constellatus
Streptococcus intermedius

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