Cloning and expression of insulin-like growth factors I and II in the shi drum (Umbrina cirrosa)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M PatrunoG Radaelli

Abstract

Insulin-like growth factors (IGFs) are evolutionarily ancient polypeptides, with potent metabolic actions, affecting cell development and growth. The IGF system consists of two ligands: IGF-I and IGF-II, several binding proteins and high-affinity transmembrane receptors. To understand growth regulation in the teleost shi drum, Umbrina cirrosa, we cloned IGF-I and IGF-II cDNAs, studied their expression and determined the cellular localization of IGF-II peptide by immunohistochemistry. A fragment of 1110 nucleotides, coding for U. cirrosa IGF-I (ucIGF-I), was cloned from liver by PCR. It includes an open reading frame of 561 nucleotides, encoding a 187 amino acid preproIGF-I. A fragment of 938 nucleotides that includes part of the coding sequence and the 3' UTR of IGF-II (ucIGF-II) was cloned as well. Sequence analysis of ucIGF-I and ucIGF-II showed a high degree of homology with known fish IGF-I and IGF-II. Real-Time PCR showed a higher expression of IGF-I and IGF-II in liver, compared to all other tissues analysed. IGF-II peptide was detected in larval liver, intestine, gills and heart musculature. After metamorphosis, reactivity was particularly evident in the kidney and in red fibres of skeletal muscle. These results add nove...Continue Reading

References

Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons
Nov 29, 1996·Molecular and Cellular Endocrinology·G EliesD Boujard
Apr 1, 1996·Journal of Molecular Endocrinology·S J DuguayS J Chan
Sep 19, 2000·Bioscience, Biotechnology, and Biochemistry·S MoriyamaH Kawauchi
May 22, 2002·Biochimica Et Biophysica Acta·Margaret C L TseKing Ming Chan
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·D G ArmstrongK J Woad
Oct 16, 2002·The Journal of Endocrinology·K M KelleyM Jamison
May 27, 2003·General and Comparative Endocrinology·Munetaka ShimizuWalton W Dickhoff
Jan 31, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Juan CastilloJoaquim Gutiérrez
May 12, 2004·Protein Expression and Purification·Ryan J WilkinsonGeoffrey Francis
Oct 7, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ryan J WilkinsonJohn Carragher
Nov 24, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brian C PetersonLelania Bilodeau
Dec 17, 2004·The International Journal of Developmental Biology·Edward EiversLucy Byrnes
Feb 11, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Thea ShavlakadzeMiranda D Grounds
Feb 11, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Nelly Mauras, Morey W Haymond

❮ Previous
Next ❯

Citations

Jan 10, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Chiara Carla PiccinettiIke Olivotto
Aug 30, 2011·General and Comparative Endocrinology·Daniela BertottoGiuseppe Radaelli
Mar 31, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sebastian EscobarAlfredo Molina
Jun 10, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Marian PonceManuel Manchado
Sep 15, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shunsuke MoriyamaHiroshi Kawauchi
Jun 4, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Andrés A SciaraSilvia E Arranz
May 4, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Carlin M FennBrian C Small
Nov 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matteo A AvellaOliana Carnevali
Jul 28, 2020·Animals : an Open Access Journal From MDPI·Antonio CarminatoDaniela Bertotto
Jan 10, 2021·Animals : an Open Access Journal From MDPI·Matteo ZarantonielloDaniela Bertotto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.