Abstract
Neuron-specific enolase (NSE) is the key molecular marker for diffuse neuroendocrine system (DNES) cells, its expression in the pregnant corpus luteum (CL) of dairy goats was studied by the immunofluorescence method and the ultra structural characteristics of luteal cells were detected by the electron microscopy to identify the existence of DNES cells in the pregnant CL of dairy goats. Besides, the coding sequence of dairy goats NSE gene was cloned and its biological information was analyzed. Results revealed that NSE immunopositive cells exhibited widespread cytoplasmic staining throughout the whole pregnant CL. In addition, these cells showed typical characteristics of DNES cells in the electron microscopy. These results suggested that many DNES cells exist in the pregnant CL of dairy goats. Meanwhile, we identified the coding sequence of dairy goats NSE (GenBank Accession No. JN887466). Its nucleotide sequence homology was found to be 97.9%, 89.3%, 90% and 92.6%, respectively, compared with that of Bos taurus, Rattus norvegicus, Mus musculus and Homo sapiens, while the amino acid sequence homology was 99.1%, 97%, 97.2% and 98.2% respectively. These results first showed that the functional amino acids coded by the NSE gene we...Continue Reading
References
Dec 21, 1978·Nature·D SchmechelM Brightman
Nov 1, 1979·The Journal of Clinical Endocrinology and Metabolism·K P McNattyK J Ryan
Nov 23, 1988·Biochimica Et Biophysica Acta·T BanasR G Kemp
Jun 16, 1988·Nature·L Lebioda, B Stec
Dec 1, 1987·The Journal of Endocrinology·S R DavisH G Burger
Jul 28, 1999·Molecular Human Reproduction·L M SenturkA Arici
Jul 12, 2000·Molecular Biology and Evolution·V HannaertW Martin
Aug 15, 2000·Digestion·B ErikssonM Stridsberg
May 5, 2001·Genome Research·P C Ng, S Henikoff
Aug 11, 2001·Cellular and Molecular Life Sciences : CMLS·V Pancholi
Aug 21, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Domenico FerrignoCristina Giordano
Sep 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marta ArchancoMaría A Burrell
Mar 19, 2004·Endocrine-related Cancer·M T BarakatS R Bloom
Jun 8, 2004·Endocrine Reviews·Gregory A KaltsasAshley B Grossman
Aug 4, 2004·Journal of Molecular Biology·Geqing ChaiLukasz Lebioda
Mar 9, 2005·Trends in Biochemical Sciences·Jung-Whan Kim, Chi V Dang
Dec 8, 2005·Domestic Animal Endocrinology·Ryosuke SakumotoKiyoshi Okuda
Aug 29, 2009·Cancer Letters·Sang-Min JangKyung-Hee Choi