Cloning and expression of somatolactin, a pituitary hormone related to growth hormone and prolactin from gilthead seabream, Sparus aurata

General and Comparative Endocrinology
Antonio AstolaM M Valdivia

Abstract

A pituitary hormone, somatolactin (SL), belonging to the GH/PRL family, is produced in the intermediate lobe of the teleost pituitary. The function of this protein is uncertain. Clones coding for SL were isolated and sequenced from a gilthead seabream pituitary cDNA expression library. The nucleotide sequence of the larger cDNA isolated was 1.5 kb containing a 0.8-kb 3'-untranslated region and two potential polyadenylation signals (AATAAA). The mature polypeptide is composed of 207 amino acids, and a signal peptide of 24 residues was also found in the SL precursor. A potential N-glycosylation site Asn-Lys-Thr was identified in gilthead seabream SL. A comparison of the SL amino acid sequences of several fishes indicated that seven cysteine residues are characteristically present in all the SLs so far isolated. Six of those residues are present in homologous positions in SL and GH Sparus aurata proteins. SL and GH from S. aurata showed a 43% homology at the nucleotide level and 22% identity at the amino acid level. Expression of recombinant SL (rSL) in Escherichia coli and isolation from inclusion bodies led to a monomeric form of SL identical in electrophoretic mobility to one of the two forms of the native SL secreted from gilt...Continue Reading

Citations

Jun 18, 1998·Annals of the New York Academy of Sciences·C PendonM M Valdivia
Mar 3, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L Vargas-ChacoffG Martínez-Rodríguez
Oct 5, 2014·General and Comparative Endocrinology·G E ValenzuelaG Kausel
Apr 20, 1999·General and Comparative Endocrinology·Y AmemiyaH Kawauchi
Dec 17, 2005·General and Comparative Endocrinology·Hiroshi Kawauchi, Stacia A Sower
Jun 27, 2003·Cell and Tissue Research·M J Herrero-TurriónJ M Lara
Feb 21, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Maximiliano Martín CánepaPaula Gabriela Vissio
Jul 11, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Quan Jiang, Anderson O L Wong
Jan 19, 2020·Thyroid : Official Journal of the American Thyroid Association·Cho Rong HanSheue-Yann Cheng
Aug 25, 2011·American Journal of Physiology. Endocrinology and Metabolism·Quan JiangAnderson O L Wong
Dec 31, 2020·The Journal of Experimental Biology·Tomás Horacio DelgadinPaula Gabriela Vissio
Jul 27, 1999·General and Comparative Endocrinology·F G AysonH Kawauchi
May 27, 2003·General and Comparative Endocrinology·M Javier Herrero-TurriónJuan M Lara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.