Abstract
Bacterial UDP-N-acetylmuramyl-L-alanine:D-glutamate ligase (MurD), a cytoplasmic peptidoglycan biosynthetic enzyme, catalyzes the ATP-dependent addition of D-glutamate to an alanyl residue of the UDP-N-acetylmuramyl-L-alanine precursor, generating the dipeptide. The murD gene was cloned from both Staphylococcus aureus and Streptococcus pyogenes. Sequence analysis of the S. aureus murD gene revealed an open reading frame of 449 amino acids. The deduced aa sequence of S. aureus MurD is highly homologous to MurD from Escherichia coli, Haemophilus influenzae, Bacillus subtilis and St. pyogenes. Recombinant MurD protein from both S. aureus and St. pyogenes was separately overproduced in E. coli and purified as His-tagged fusion. Both recombinant enzymes catalyzed the ATP-dependent addition of D-glutamate to the precursor sugar peptide.
References
Jul 1, 1992·Biochimie·A O HenriquesP J Piggot
Dec 18, 1991·European Journal of Biochemistry·F Pratviel-SosaJ van Heijenoort
Jan 11, 1990·Nucleic Acids Research·D Mengin-Lecreulx, J van Heijenoort
Feb 25, 1990·Nucleic Acids Research·M IkedaM Matsuhashi
Jul 11, 1990·Nucleic Acids Research·M IkedaM Matsuhashi
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 11, 1989·Nucleic Acids Research·C ParquetJ van Heijenoort
Nov 1, 1989·Canadian Journal of Microbiology·J S Tao, E E Ishiguro
Aug 3, 1987·European Journal of Biochemistry·C MichaudJ Van Heijenoort
Apr 1, 1972·Journal of Bacteriology·E J Lugtenberg
Oct 16, 1995·Gene·S L RowlandR G Wake
Feb 1, 1993·Journal of General Microbiology·R A Daniel, J Errington
Mar 1, 1993·Microbiological Reviews·J Errington
Feb 1, 1996·Journal of Bacteriology·M GublerW Keck
Feb 2, 1996·Gene·Y GeN W Charon
Feb 6, 1996·Biochemistry·H JinJ J Villafranca
May 20, 1997·Biochemistry·S S EvelandM S Anderson
Sep 1, 1997·Journal of Bacteriology·M J PucciT J Dougherty
Sep 19, 1997·Journal of Bacteriology·H HaraJ T Park
Oct 8, 1997·Biochemistry·A BouhssC Parquet
Jan 1, 1997·Journal of Molecular Evolution·H WatanabeT Gojobori
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J FerrettiR McLaughlin
Mar 8, 1996·The Journal of Organic Chemistry·Martin E. TannerDidier Blanot
Citations
Jul 27, 2001·FEMS Microbiology Letters·A El ZoeibyR C Levesque
Dec 11, 1999·Biochimica Et Biophysica Acta·E Breukink, B de Kruijff
Apr 3, 2001·Protein Expression and Purification·B A AzzolinaM El-Sherbeini
Feb 17, 2000·FEMS Microbiology Letters·A El ZoeibyR C Levesque
Sep 21, 2019·Journal of Receptor and Signal Transduction Research·Srikanth JupudiAshish Wadhwani
Dec 2, 2014·Biomolecular Concepts·Roman ŠinkDidier Blanot
Aug 28, 1999·Journal of Bacteriology·A W WalshH T Ho
May 7, 2020·IJC Heart & Vasculature·Chenglin YuXian Wu Cheng