PMID: 8950170Nov 11, 1996Paper

Cloning and expression of the cDNA for mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase

Biochimica Et Biophysica Acta
M MatsuoH H Tai

Abstract

The cDNA for mouse NAD+ dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) was isolated from a lung cDNA library. The cDNA contains a 798 bp open reading frame that codes for a protein of 266 amino acids (M(r) 28775) which shares 87% identity with the human 15-PGDH protein. The regions that are believed to form the NAD+ binding site and the active site are conserved in the mouse and human enzymes. The authenticity of the mouse cDNA was confirmed by expression of an active 15-PGDH in Escherichia coli. Northern blot analysis demonstrated that 15-PGDH mRNA is expressed primarily in lung, intestine, stomach and liver.

References

Oct 1, 1976·Prostaglandins·H S Hansen
Oct 15, 1992·Biochemical and Biophysical Research Communications·J Obeid, P C White
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K I VarugheseN H Xuong
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D GhoshJ C Orr
May 9, 1995·Biochemistry·H JörnvallD Ghosh

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Citations

Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Hsin-Hsiung TaiFengxiang Yan
Aug 30, 2003·American Journal of Respiratory Cell and Molecular Biology·Michael A ShultzAlan R Buckpitt
Dec 29, 2000·The Journal of Trauma·E L Hahn, R L Gamelli
Mar 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Marianne ParentMichel A Fortier
Dec 7, 2007·American Journal of Physiology. Renal Physiology·Bing YaoMing-Zhi Zhang
Dec 23, 1999·The Journal of Trauma·E L HahnR L Gamelli
Mar 11, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Hiroaki ShimadaMasahiro Iwaki

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