PMID: 8590305Nov 1, 1995Paper

Cloning and expression of the chicken interferon-gamma gene

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
M Digby, J W Lowenthal

Abstract

We have cloned the gene for chicken interferon-gamma (ChIFN-gamma) from a cDNA expression library generated from a T cell line (CC8.1h) that produces high levels of IFN-gamma activity. CC8.1h constitutively produces IFN activity that shares physiochemical properties with mammalian IFN-gamma. ChIFN-gamma, when secreted by CC8.1h or expressed in transfected COS cells, is heat labile, inactivated by exposure to pH 2, and capable of inducing nitrite production by chicken macrophages. These properties clearly distinguish it from chicken and mammalian type I IFN. The ChIFN-gamma gene codes for a predicted mature protein of 145 amino acids with a molecular mass of 16.8 kD. There are two potential N-glycosylation sites located near the N terminus. ChIFN-gamma protein shares significant amino acid homology with mammalian IFN-gamma proteins; in particular it also contains the highly conserved motifs that are present in all mammalian IFN-gamma proteins. ChIFN-gamma is 35 and 32% identical to the equine and human counterparts, respectively, but shares only 15% homology with chicken type I IFN. These findings show that the emergence of the two principal types of IFN predates the divergence of avians and mammals that occurred some 350 millio...Continue Reading

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Citations

Jun 2, 2012·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·A K Tewari, B R Maharana
Apr 6, 2004·Developmental and Comparative Immunology·Ursula SchultzPeter Staeheli
Apr 28, 2000·Developmental and Comparative Immunology·G A Harrison, D N Wedlock
Sep 12, 2000·Developmental and Comparative Immunology·A HuangI Kotlarski
Jul 27, 2001·Developmental and Comparative Immunology·T V Leshchinsky, K C Klasing
Apr 17, 2003·Developmental and Comparative Immunology·Tawni L CrippenMichael H Kogut
Dec 1, 1999·Developmental and Comparative Immunology·S RautenschleinJ M Sharma
Feb 26, 2000·Developmental and Comparative Immunology·C Siatskas, R Boyd
Mar 16, 2000·Developmental and Comparative Immunology·D L Suarez, S Schultz-Cherry
Mar 16, 2000·Developmental and Comparative Immunology·M A JohnsonJ W Lowenthal
Mar 16, 2000·Developmental and Comparative Immunology·J W LowenthalA G Bean
Mar 14, 2000·Veterinary Immunology and Immunopathology·C H YunK D Choi
Dec 4, 2001·Veterinary Immunology and Immunopathology·L RothwellP Kaiser
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