Cloning and expression of the gene for a vanadium-dependent bromoperoxidase from a marine macro-alga, Corallina pilulifera

FEBS Letters
M ShimonishiT Tanabe

Abstract

The cDNAs for a vanadium-dependent bromoperoxidase were cloned from a marine macro-alga, Corallina pilulifera. The open reading frame of one clone (bpo1) encoded a protein of 598 amino acids with a calculated molecular mass of 65312 Da in good agreement with that of 64 kDa determined for the native enzyme. The deduced amino acid sequence coincided well with partial sequences of peptide fragments of the enzyme. From the same cDNA library we also isolated another cDNA clone (bpo2) encoding a protein of 597 amino acids with an identity of about 90% to BPO1, suggesting a genetic diversity of the bromoperoxidase gene of C. pilulifera growing in a relatively narrow area. The carboxy-terminal 123 residues of the enzyme (BPO1) showed an identity of 45% to that of the marine macro-alga Ascophillum nodosum. The homology search of the sequences of bromoperoxidases from C. pilulifera (this study) and A. nodostum, and chloroperoxidase from the fungus Curvularia inaequalis indicated highly conserved sequences PxYxSGHA and LxxxxAxxRxxxGxHxxxD. Furthermore, it was found that the histidine residue directly bound to vanadium, other residues building up the metal center and catalytic histidine residue forming the active site of the chloroperoxida...Continue Reading

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Citations

Nov 26, 2002·Journal of Molecular Recognition : JMR·Jennifer LittlechildMisha Isupov
Jan 29, 2002·Archives of Biochemistry and Biophysics·Takafumi KawanamiNobuya Itoh
Mar 19, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Esther Garcia-RodriguezJennifer Littlechild
Jul 18, 2002·Journal of Inorganic Biochemistry·Jayme N CarterAlison Butler
Feb 18, 1999·Current Opinion in Chemical Biology·J Littlechild
Apr 14, 2009·The Journal of Biological Chemistry·Jaclyn M Winter, Bradley S Moore
May 11, 2004·Protein Science : a Publication of the Protein Society·Takashi OhshiroYoshikazu Izumi
Aug 19, 2014·Bioscience, Biotechnology, and Biochemistry·Kensuke KanekoTatsufumi Okino
Feb 24, 2009·Journal of Inorganic Biochemistry·Jennifer LittlechildMikhail Isupov
Apr 25, 2018·Marine Biotechnology·Thillai PunithaJohn Beardall
Apr 17, 2003·The Journal of Biological Chemistry·Carole ColinPhilippe Potin
Jul 25, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Diana WischangJens Hartung
Jun 29, 1999·Current Opinion in Microbiology·P PotinB Kloareg
May 29, 2002·Chemistry & Biology·Brenda ValderramaRafael Vazquez-Duhalt

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