Abstract
Manganese superoxide dismutase (Mn-SOD; SOD(2)) is an important antioxidant defense enzyme. In this study, a full-length Mn-SOD cDNA was cloned from the cDNA library of Phascolosoma esculenta. The cDNA is 1385 bp in length, including an open reading frame (ORF) of 681 bp encoding 226 amino acids. The predicted protein has a calculated molecular weight of 25.2 kDa and a theoretical isoelectric point of 5.96. BLAST analysis revealed the predicted protein shared 70% identity with homologues in Caenorhabditis elegans and Gallus gallus. The SOD gene was inserted into Escherichia coli expression plasmid pET-28a (+) to produce pET-SOD. The recombinant Mn-SOD of P. esculenta was expressed following IPTG induction, and verified by Western blot analysis using antiserum from immunized mice. Furthermore, fluorescent real-time PCR analysis revealed varying degrees of induction of SOD(2) mRNA expression in the blood of P. esculenta exposed to heavy metals (Cd(2+), Cu(2+) and Zn(2+)) and thermal stresses (4 degrees C and 37 degrees C).
Citations
Aug 8, 2012·Genetics and Molecular Research : GMR·C H LiT W Li
Jan 28, 2014·Aquatic Toxicology·Kai LyuZhou Yang
Jul 26, 2014·Applied Biochemistry and Biotechnology·Chaomin YinAimin Ma
Sep 4, 2014·Fish Physiology and Biochemistry·Jesu ArockiarajMarimuthu Kasi
Jan 19, 2013·Environmental Toxicology and Pharmacology·Ren-Kuan LiXiu-Yun Ye
Jan 15, 2013·Gene·Jianghua YangJiaxin Yang
Dec 28, 2010·Developmental and Comparative Immunology·Maki OhtaniTakashi Aoki
Sep 24, 2015·Fish & Shellfish Immunology·Mengqiang WangLinsheng Song
Sep 28, 2015·Gene·Jiao LiuBin Zhou
Nov 26, 2013·Journal of Insect Physiology·Haihong JiaBaohua Xu
Mar 29, 2011·Veterinary Immunology and Immunopathology·You Chuan WeiP Nie
Mar 1, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Chenghua LiTaiwu Li
Feb 6, 2015·Fish & Shellfish Immunology·Huihui LiuYifeng Gu
Jan 9, 2013·International Journal of Molecular Sciences·Xian-Long GaoXiao-Wei Wang
Jul 21, 2017·Protein Science : a Publication of the Protein Society·Hongwei DingXiurong Su
May 5, 2011·Archives of Insect Biochemistry and Physiology·Jun-Min LiXiao-Wei Wang
Feb 3, 2021·Ecotoxicology and Environmental Safety·Tinghong MingXiurong Su
Aug 29, 2021·Molecular Biology Reports·Jian-Dong ZhangGang Chen