PMID: 9178820May 16, 1997Paper

Cloning and expression of the recombinant FAb fragment of monoclonal antibody K1 that reacts with mesothelin present on mesotheliomas and ovarian cancers

International Journal of Cancer. Journal International Du Cancer
U BrinkmannI Pastan

Abstract

The monoclonal antibody (MAb) K1 recognizes an approximate 40 kDa glycoprotein, mesothelin, that is present on the surface of human mesothelial cells, mesotheliomas and ovarian cancers. We have cloned the cDNAs encoding the variable regions of MAb K1 and constructed plasmids for expression of recombinant K1(FAb). Recombinant FAb was produced in Escherichia coli in inclusion bodies that were solubilized and refolded to active protein. Binding of K1 MAb and FAb was compared by radioactive binding and competition assays and by surface plasmon resonance (BIAcore). Recombinant K1(FAb) binds to cells expressing K1-antigen with a similar affinity as papain derived FAb from K1(IgG) and with a 4- to 10-fold reduced affinity compared with bivalent IgG. The cloned FAb can be used to make higher affinity antibodies and immunoconjugates that could be useful for various types of immunotherapies.

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Citations

Sep 8, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Junko YokokawaKwong Y Tsang
Oct 14, 2009·Seminars in Thoracic and Cardiovascular Surgery·Harvey I Pass, Michele Carbone
Aug 18, 2009·Biomarkers in Medicine·Dimitra SasaroliNathalie Scholler
Jul 5, 2005·Expert Opinion on Investigational Drugs·S MukerjeeM C Glassy
Sep 22, 2007·Advanced Drug Delivery Reviews·David Filpula, Hong Zhao

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