PMID: 11900958Mar 20, 2002Paper

Cloning and expression of the superoxide dismutase gene of the feline strain of Porphyromonas gingivalis: immunological recognition of the protein by cats with periodontal disease

Veterinary Microbiology
Daria N LoveJacqueline M Norris

Abstract

Recent evidence suggests that feline members of the genus Porphyromonas are of consequence in periodontal disease in cats. Several possible virulence factors from feline strains of Porphyromonas gingivalis have been described that have similarities to those of human P. gingivalis. Both human and feline strains of P. gingivalis produce superoxide dismutase (SOD) which has been proposed as modulator of the inflammatory response during infection. The objective of this study was to clone the superoxide dismutase gene of feline P. gingivalis, to compare the characteristics of its product with that of the native enzyme and to determine its immunoreactivity in cats with periodontal disease. The sod gene of the feline strain Veterinary Pathology and Bacteriology (VPB) 3457 of P. gingivalis was amplified by PCR and cloned in frame with the alpha-peptide of the LacZ gene of E. coli in plasmid pUC19. This construct expressed SOD activity in E. coli with characteristics similar to those of the native SOD enzyme of P. gingivalis human strain 381 and the parent feline strain VPB 3457. The recombinant SOD had an apparent molecular weight of 54,700+/-1300 (S.E.M.) and was inactivated by 5mM hydrogen peroxide but not by 2mM KCN. There was a sig...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·J D CrapoI Fridovich
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May 1, 1978·Applied and Environmental Microbiology·E M GregoryL V Holdeman
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Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith

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Citations

Nov 7, 2015·Current Microbiology·Hebin LiaoAnchun Cheng
Jun 10, 2016·Microbiome·Christina J AdlerJacqueline M Norris

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