Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii

Journal of Bacteriology
A AlmagroJosé Ramos

Abstract

Two genes encoding Na(+)-ATPases from Debaryomyces hansenii were cloned and sequenced. The genes, designated ENA1 from D. hansenii (DhENA1) and DhENA2, exhibited high homology with the corresponding genes from Schwanniomyces occidentalis. DhENA1 was expressed in the presence of high Na(+) concentrations, while the expression of DhENA2 also required high pH. A mutant of Saccharomyces cerevisiae lacking the Na(+) efflux systems and sensitive to Na(+), when transformed with DhENA1 or DhENA2, recovered Na(+) tolerance and also the ability to extrude Na(+).

References

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Jan 1, 1993·Molecular & General Genetics : MGG·B GarciadeblasA Rodríguez-Navarro
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Jun 17, 2000·International Journal of Food Microbiology·A AlmagroM C Loureiro-Dias

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Citations

Oct 22, 2005·Applied Microbiology and Biotechnology·H D MortensenN Arneborg
Jul 14, 2009·Extremophiles : Life Under Extreme Conditions·Sawan Kumar, Sathyanarayana N Gummadi
Jun 10, 2005·FEMS Yeast Research·Catarina PristaJosé Ramos
Nov 10, 2009·Applied and Environmental Microbiology·Marjetka Kralj KuncicNina Gunde-Cimerman
Oct 24, 2007·Eukaryotic Cell·Amparo Ruiz, Joaquín Ariño
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Joaquín AriñoHana Sychrová
Jun 29, 2014·International Journal of Food Microbiology·Tikam Chand DakalPaolo Giudici
Sep 15, 2009·Mycological Research·Nina Gunde-CimermanAna Plemenitas
Jul 3, 2007·International Journal of Food Microbiology·Klara Papouskova, Hana Sychrova
Jul 24, 2010·Biochimica Et Biophysica Acta·Alonso Rodríguez-Navarro, Begoña Benito
May 27, 2014·Frontiers in Microbiology·Ana PlemenitašNina Gunde-Cimerman
Aug 26, 2009·FEMS Yeast Research·Martha CalahorraAntonio Peña
Aug 30, 2008·FEMS Yeast Research·Norma Silvia SánchezAntonio Peña
Mar 3, 2007·FEMS Yeast Research·Klaus GoriLene Jespersen
Jan 19, 2011·FEMS Microbiology Letters·José RamosHana Sychrová
Apr 19, 2003·FEMS Yeast Research·Catarina PristaMaria C Loureiro-Dias
Mar 24, 2009·FEMS Yeast Research·Clara NavarreteJosé Ramos
Feb 8, 2014·PloS One·Iliana PereiraMaria José Leandro
Sep 8, 2018·Yeast·Joaquín AriñoHana Sychrova
Apr 5, 2002·Microbiology·Begoña BenitoAlonso Rodríguez-Navarro
May 29, 2018·Fungal Biology·Drauzio E N RangelGeoffrey Michael Gadd

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