Cloning and expression pattern of facilitative glucose transporter 1 (GLUT1) in gilthead sea bream Sparus aurata in response to salinity acclimation

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
C Balmaceda-AguileraGonzalo Martínez-Rodríguez

Abstract

Facilitative glucose transporters (GLUT) are transmembrane transporter proteins involved in glucose transport across the plasma membrane. In fish, several GLUT mRNAs have been cloned, but to date there is no information about these transporters in the marine euryhaline teleost Sparus aurata. In the present study we obtained the complete coding sequence from S. aurata GLUT1 (saGLUT1), composed by 4483 bases, presenting a 79 to 95% identity with respect to other fish GLUT1 mRNAs. The analysis of the 5' and 3' UTRs showed the presence of several post-transcriptional regulatory elements. In addition, the effect on saGLUT1 mRNA expression of the osmotic acclimation to four different environmental salinities (5, 12, 40 and 55 ppt), in gills, kidney, liver and brain, was studied. Changes in mRNA expression levels were detected in gills and brain, indicating that GLUT1 has an important role in these organs for osmotic acclimation in S. aurata.

References

Feb 1, 1994·European Journal of Biochemistry·M Mueckler
Mar 16, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H TeerijokiH Mölsä
Aug 22, 2001·Biochimica Et Biophysica Acta·A KrasnovH Mölsä
Oct 9, 2001·Bioinformatics·G E Tusnády, I Simon
Apr 18, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·José L Soengas, Manuel Aldegunde
May 17, 2003·Pflügers Archiv : European journal of physiology·Marc Uldry, Bernard Thorens
Jun 21, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Susana Sangiao-AlvarellosJosé L Soengas
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Klaus W Beyenbach
Dec 8, 2004·The Journal of Experimental Biology·Jennifer R HallWilliam R Driedzic
Jun 10, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Raúl Laiz-CarriónJuan M Mancera
Aug 6, 2005·Biochimica Et Biophysica Acta·Jennifer R HallWilliam R Driedzic
Nov 8, 2005·The Journal of Experimental Biology·Susana Sangiao-AlvarellosJosé L Soengas
Jan 25, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Sergio PolakofJosé L Soengas
Jun 10, 2006·The EMBO Journal·Amanda CharlesworthAngus M MacNicol
Jan 24, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Genciana TerovaMarco Saroglia
May 22, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yung-Che TsengPung-Pung Hwang
Aug 5, 2009·General and Comparative Endocrinology·Olga HrytsenkoJames R Wright
Aug 5, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Luis Vargas-ChacoffJuan M Mancera
Sep 25, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Juan CastilloJosep V Planas
Dec 17, 2009·American Journal of Physiology. Endocrinology and Metabolism·Bernard Thorens, Mike Mueckler
Jan 22, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marija MihailovichFátima Gebauer
Aug 21, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Klaus WethmarAchim Leutz
Jan 5, 2011·Annals of Neurology·Ping-Pin ZhengJohan M Kros

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Citations

Sep 12, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J A Martos-SitchaG Martínez-Rodríguez
Sep 30, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·José Antonio Mata-SotresGonzalo Martínez-Rodríguez
Aug 5, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Juan A Martos-SitchaGonzalo Martínez-Rodríguez
Aug 30, 2014·Molecular Biology Reports·José A Martínez-QuintanaGloria Yepiz-Plascencia
Sep 28, 2013·The Journal of Experimental Biology·Alison M GardellDietmar Kültz

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