PMID: 9194573May 27, 1997Paper

Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor

Biochimica Et Biophysica Acta
Perry BarrettP Morgan

Abstract

We have isolated a novel variant of the Mel 1a melatonin receptor from an ovine PT cDNA library. Relative to the reported sequence for the Mel 1a melatonin receptor there are 8 changes in the DNA sequence. Only 3 of these result in amino acid substitutions, one in extracellular loop 3 and two in the carboxy-terminal tail. We have designated the novel variant of the sheep Mel 1a receptor Mel 1a(beta), and correspondingly the previously reported variant Mel 1a(alpha). As minor changes in the primary amino acid sequence of G-protein-coupled receptors can influence their functional characteristics we have accordingly characterized this novel variant of the Mel 1a melatonin receptor. This melatonin receptor displays high affinity binding and inhibits the cAMP second messenger pathway in transfected L-cells demonstrating that this receptor is fully functional. PCR analysis shows Mel 1a(beta) is present in several breeds of sheep and suggests that the Mel 1a(beta) receptor was established early in the evolution of the sheep species.

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Citations

Mar 10, 2000·European Journal of Pharmacology·S ConwayP J Morgan
Apr 26, 2003·General and Comparative Endocrinology·Masafumi AmanoKunio Yamamori
Mar 12, 2003·Life Sciences·Paula A Witt-EnderbyMelissa A Melan
May 1, 2001·Journal of Neuroendocrinology·J E DrewP J Morgan
Oct 3, 2003·Journal of Pineal Research·Perry BarrettPeter J Morgan
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·D R Notter
Apr 4, 2008·Biochemical Pharmacology·Valérie AudinotJean A Boutin
May 29, 2013·International Journal of Molecular Sciences·Di Yan LiQing Zhu
Jan 8, 2011·Reproduction, Fertility, and Development·Vincenzo CarcangiuMaria Consuelo Mura
Apr 29, 1998·Annals of Medicine·T Kokkola, J T Laitinen
Nov 21, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J M FustinD G Hazlerigg
Sep 23, 1998·Journal of Neuroendocrinology·J E DrewP J Morgan
Dec 6, 2019·Frontiers in Endocrinology·Lei LiuRalf Jockers
Jun 20, 2002·Journal of Pineal Research·Pascaline GaildratJack Falcón
Nov 5, 1997·Biochemical and Biophysical Research Communications·S ConwayP J Morgan
Sep 2, 1999·Biochemical and Biophysical Research Communications·T EbisawaT Yamauchi
Nov 9, 2007·Journal of Medicinal Chemistry·Andrew TsotinisDavid Sugden

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