Cloning and functional analysis of FLJ20420: a novel transcription factor for the BAG-1 promoter.

PloS One
Hongyu LiuJun Chen

Abstract

BAG-1 is an anti-apoptotic protein that interacts with a variety of cellular molecules to inhibit apoptosis. The mechanisms by which BAG-1 interacts with other proteins to inhibit apoptosis have been extensively explored. However, it is currently unknown how BAG-1 expression is regulated at the molecular level, especially in cancer cells. Here we reported to clone a novel down-regulated BAG-1 expression gene named FLJ20420 using hBAG-1 promoter as a probe to screen Human Hela 5' cDNA library by Southernwestern blot. The FLJ20420 gene encodes a ∼26-kDa protein that is localized in both the cytoplasm and nucleus. We proved that FLJ20420 protein can specially bind hBAG-1 promoter region by EMSA in vivo and ChIP assay in vivo. Northern blot analysis revealed a low level of FLJ20420 transcriptional expression in normal human tissues (i.e., brain, placenta, lung, liver, kidney, pancreas and cervix), except for heart and skeletal muscles, which showed higher levels. Furthermore, enhanced FLJ20420 expression was observed in tumor cell lines (i.e., MDA468, BT-20, MCF-7, C33A, HeLa and Caski). Knockdown of endogenous FLJ20420 expression significantly increased BAG-1 expression in A549 and L9981 cells, and also significantly enhanced thei...Continue Reading

References

Aug 15, 1997·The EMBO Journal·S TakayamaJ C Reed
Nov 5, 1997·Biochemical and Biophysical Research Communications·M SekiyaK Imai
Aug 26, 1998·Leukemia & Lymphoma·M AdachiK Imai
Jan 5, 1999·The Journal of Biological Chemistry·S TakayamaJ C Reed
Feb 27, 1999·Experimental Cell Research·X YangA Pater
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M PerouD Botstein
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S C TangN Shaheta
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R C LiR Bolli
Aug 9, 2001·International Journal of Cancer. Journal International Du Cancer·S RorkeS C Tang
Jun 7, 2002·IUBMB Life·Shou-Ching Tang
Oct 27, 2004·Molecular & Cellular Proteomics : MCP·Kei FukadaHaining Zhu
Jan 14, 2005·Molecular Biology of the Cell·George B JohnJiping Zha
Dec 13, 2005·Genesis : the Journal of Genetics and Development·Jun ChenNian Zhang
Mar 28, 2008·Genes, Brain, and Behavior·L UrigüenJ J Meana
Apr 29, 2008·Biochemical and Biophysical Research Communications·Wei MaQinghua Zhou
Dec 29, 2009·Acta Pharmacologica Sinica·Fan ZhangQing-hua Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
competition binding
ChIP
immunoprecipitation
PCR
transfection
Luciferase
FACS
flow cytometry
myristylation
surgical resection

Software Mentioned

BLAST ( Basic Local Alignment Search Tool )

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.