Cloning and functional analysis of two type 1 diacylglycerol acyltransferases from Vernonia galamensis

Phytochemistry
Keshun YuDavid Hildebrand

Abstract

Vernonia galamensis accumulates vernolic acid (cis-12-epoxyoctadeca-cis-9-enoic acid) as the major fatty acid in its seed oil. Such epoxy fatty acids are useful in a number of industrial applications. Successful genetic engineering of commercial oilseed crops to produce high levels of vernolic acid depends on a better understanding of the source plant enzymes for vernolic acid accumulation. Developing V. galamensis seed microsome assays demonstrate that diacylglycerol acyltransferase (DGAT), an enzyme for the final step of triacylglycerol synthesis, has a strong substrate preference for vernolic acid bearing substrates including acyl-CoA and diacylglycerol. There are two classes of DGATs known as DGAT1 and DGAT2. Here we report on the isolation, characterization, and functional analysis of two DGAT1 cDNAs from V. galamensis (VgDGAT1a and VgDGAT1b). VgDGAT1a and VgDGAT1b are expressed in all plant tissues examined with highest expression in developing seeds. Enzymatic assays using isolated microsomes from transformed yeast show that VgDGAT1a and VgDGAT1b have the same DGAT activity levels and substrate specificities. Oleoyl-CoA and sn-1,2-dioleoylglycerol are preferred substrates over vernoloyl-CoA and sn-1,2-divernoloylglycerol...Continue Reading

References

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Citations

Jan 17, 2013·Functional & Integrative Genomics·Runzhi LiDavid Hildebrand
Jun 19, 2012·Progress in Lipid Research·Qin LiuRandall J Weselake
Jan 19, 2010·Plant Biotechnology Journal·Runzhi LiDavid F Hildebrand
Sep 1, 2016·Journal of Agricultural and Food Chemistry·Tomoko HatanakaDavid Hildebrand
Oct 6, 2016·Genetics and Molecular Biology·Andreia Carina Turchetto-ZoletMarcia Margis-Pinheiro
Feb 13, 2018·Plant Reproduction·Sébastien Baud

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