Cloning and functional expression in Escherichia coli of the gene encoding the di- and tripeptide transport protein of Lactobacillus helveticus.

Applied and Environmental Microbiology
H NakajimaW N Konings

Abstract

The gene encoding the di- and tripeptide transport protein (DtpT) of Lactobacillus helveticus (DtpTLH) was cloned with the aid of the inverse PCR technique and used to complement the dipeptide transport-deficient and proline-auxotrophic Escherichia coli E1772. Functional expression of the peptide transporter was shown by the uptake of prolyl-[14C] alanine in whole cells and membrane vesicles. Peptide transport via DtpT in membrane vesicles is driven by the proton motive force. The system has specificity for di- and tripeptides but not for amino acids or tetrapeptides. The dtpTLH gene consists of 1,491 bp, which translates into a 497-amino-acid polypeptide. DtpTLH shows 34% identity to the di- and tripeptide transport protein of Lactococcus lactis and is also homologous to various peptide transporters of eukaryotic origin, but the similarity between these proteins is confined mainly to the N-terminal halves.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Aug 1, 1991·Molecular Microbiology·C MathiopoulosA L Sonenshein
Jun 1, 1995·Molecular Microbiology·H Y SteinerJ M Becker
Nov 1, 1995·The Journal of Dairy Research·M SasakiP S Tan
Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Jun 1, 1990·Applied and Environmental Microbiology·M DelleyH Hottinger

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Citations

Feb 20, 2004·Journal of Bacteriology·Ulrike SamenDieter J Reinscheid
May 13, 2004·The Journal of Biological Chemistry·Chien-Sung ChiangYi-Fang Tsay
Feb 28, 2014·Nature·Joanne L Parker, Simon Newstead
Feb 2, 2000·Applied and Environmental Microbiology·K Savijoki, A Palva
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Christian A BippesDaniel J Müller
Apr 22, 2006·Applied Microbiology and Biotechnology·Kirsi SavijokiPekka Varmanen
Nov 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Joanne L ParkerSimon Newstead
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marek KieliszekAnna M Kot
Sep 29, 2009·Journal of Molecular Biology·Fabio CasagrandeDimitrios Fotiadis

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