Cloning and functional expression of the human type 1 sigma receptor (hSigmaR1)

Biochemical and Biophysical Research Communications
R KekudaV Ganapathy

Abstract

We have isolated a cDNA clone from a human placental choriocarcinoma cell (JAR) cDNA library which codes for a functional type 1 sigma receptor. An RT-PCR product obtained from guinea pig kidney mRNA using primers specific for the guinea pig sigma receptor cDNA was used to screen the cDNA library. The hSigmaR1 cDNA predicts a protein of 223 amino acids with a single putative transmembrane domain. The amino acid sequence exhibits 93% identity with the guinea pig sigma receptor. When functionally expressed in HeLa cells, the hSigmaR1 cDNA enhances the binding of [3H]-haloperidol, a sigma receptor ligand, to the HeLa cell membranes. The inhibitor specificity of the cloned hSigmaR1 indicates that it is the type 1 sigma receptor. Several human tissues, including placenta, liver, and brain, and several human cell lines express the SigmaR1 mRNA (1.7 kb) to a variable extent.

Citations

Nov 1, 2001·Brain Research. Molecular Brain Research·M S OlaS B Smith
Apr 6, 2002·British Journal of Pharmacology·Anis KlouzDidier Morin
May 25, 2002·The European Journal of Neuroscience·Vân-Ly PhanTangui Maurice
Mar 21, 2002·Fundamental & Clinical Pharmacology·Laurent Monassier, Pascal Bousquet
Aug 10, 2004·Psychiatry and Clinical Neurosciences·Fumiaki SatohHiroshi Suwaki
Nov 10, 2001·Annual Review of Genomics and Human Genetics·R I Kelley, G E Herman
Nov 19, 2010·American Journal of Physiology. Cell Physiology·Molly JohannessenMeyer B Jackson
Mar 13, 2009·American Journal of Physiology. Cell Physiology·Molly JohannessenMeyer B Jackson
May 13, 2008·Investigative Ophthalmology & Visual Science·Sylvia B SmithVadivel Ganapathy
Apr 26, 2007·Anesthesia and Analgesia·Sufang YangJeffrey R Kirsch
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Kazuhiro WatanabeJuichi Sato
Apr 19, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Qing-Xia XuLi-Yan Xu
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·M P Morin-SurunF P Monnet
Jan 6, 2011·Expert Opinion on Therapeutic Targets·Md Shenuarin Bhuiyan, Kohji Fukunaga
Dec 9, 2015·Current Eye Research·Arul K ShanmugamSylvia B Smith
Jan 18, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Athanasia DasargyriJean-Christophe Leroux
Apr 19, 2016·Journal of Drug and Alcohol Research·Yuko Yasui, Tsung-Ping Su
Aug 23, 2012·Journal of Pharmaceutical and Biomedical Analysis·James A FishbackRae R Matsumoto
Dec 20, 2015·European Journal of Pharmacology·Betzabeth Anali García-MartínezFrancisco Javier López-Muñoz
Oct 12, 2010·Life Sciences·Vadivel Ganapathy
Sep 28, 2010·Trends in Pharmacological Sciences·Tsung-Ping SuArnold E Ruoho
Jun 16, 2010·European Journal of Pharmacology·Masahiro OhsawaJunzo Kamei
Jan 7, 2004·Biological Psychiatry·Ryosuke MiyatakeHiroshi Suwaki
Aug 15, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Véronique MégalizziRobert Kiss
Jan 5, 2010·PET Clinics·Robert H MachKenneth T Wheeler
Dec 11, 2007·Neurochemistry International·Michael SchumacherEtienne-Emile Baulieu
Jul 31, 2007·Pharmacology & Therapeutics·Michael SchumacherAlejandro F De Nicola

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