Cloning and functional expression of the luteinizing hormone receptor complementary deoxyribonucleic acid from the marmoset monkey testis: absence of sequences encoding exon 10 in other species

Endocrinology
F P ZhangI T Huhtaniemi

Abstract

Based on sequence homologies among the human, porcine, rat, and mouse genes for the LH receptor (LHR), overlapping partial fragments of LHR complementary DNAs (cDNAs) were multiplied from marmoset monkey testicular RNA using reverse transcription-PCR. Ligations of the individual cDNA fragments generated a full-length monkey LHR cDNA (2031 bp) containing the complete amino acid-coding sequence (676 amino acids). Northern hybridization analysis of monkey testicular RNA, using a complementary RNA probe corresponding to the full-length cDNA, demonstrated major transcripts of 5.5 and 1.4 kilobases and minor ones of 4.0, 2.7, and 1.9 kilobases. Sequence analysis of the monkey LHR cDNA revealed a striking feature, i.e. the absence of an 81-bp nucleotide sequence corresponding to exon 10, present in the LHR cDNAs of all other species studied to date. The monkey LHR cDNA displayed 83-94% overall sequence homology with the other mammalian LHR cDNAs. Reverse transcription-PCR with human exon 10-specific primers demonstrated the total absence of this sequence from the monkey LHR messenger RNA. Southern hybridization of monkey genomic DNA using a human exon 10 probe demonstrated its presence in the monkey gene and that it is totally spliced...Continue Reading

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Citations

Feb 25, 2003·Toxicologic Pathology·U Zühlke, G Weinbauer
Sep 30, 2005·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Ling-Hong LiMari S Golub
Apr 12, 2002·Endocrine Reviews·Mario AscoliDeborah L Segaloff
May 21, 2013·Human Reproduction Update·Britta TroppmannJörg Gromoll
May 17, 2007·Molecular Endocrinology·Jörg GromollManuela Simoni
Aug 12, 2004·The Journal of Biological Chemistry·William R MoyleMichael P Bernard
Nov 4, 2006·Molecular and Cellular Endocrinology·M Ram Sairam, P Suresh Babu
Dec 7, 2010·General and Comparative Endocrinology·Audrey A VasauskasJonathan G Scammell

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