PMID: 8955320Dec 1, 1996Paper

Cloning and genetic characterization of Helicobacter pylori catalase and construction of a catalase-deficient mutant strain

Journal of Bacteriology
S OdenbreitR Haas

Abstract

The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. pylori catalase. The gene, designated katA, comprises 1,518 nucleotides and encodes a putative protein of 505 amino acids with a predicted Mr of 58,599. A second open reading frame, orf2, encoding a putative 32,715-Da protein of unknown function, follows katA. The transcriptional start site of katA mRNA was determined, but no typical consensus promoter sequence was present. A potential binding site for the Fur protein is located upstream of katA. When introduced into the catalase-deficient E. coli double-mutant UM255, the cloned gene readily complemented E. coli for catalase activity. H. pylori KatA is highly homologous to catalases in both prokaryotes and eukaryotes, with the highest homology being shown to Bordetella pertussis (64.9%), Bacteroides fragilis (59.8%), and Haemophilus influenzae (57.9%) catalases. Transposon insertion mutants were generated in three independent H. pylori strains by TnMax5-mediated tran...Continue Reading

References

Jul 26, 1977·Archives of Microbiology·M Rupprecht, K H Schleifer
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1992·Molecular Microbiology·H LeyingR Haas
Oct 17, 1991·The New England Journal of Medicine·A NomuraM J Blaser
Jan 1, 1991·Journal of Bacteriology·I von OssowskiP C Loewen
Jul 29, 1989·Lancet·C A LingwoodB Drumm
Sep 1, 1988·Journal of Bacteriology·B L Triggs-RaineP C Loewen
Aug 1, 1987·Journal of Bacteriology·P C Loewen, J Switala
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jul 15, 1988·Biochemical and Biophysical Research Communications·A Heimberger, A Eisenstark
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Sep 1, 1995·FEMS Microbiology Letters·H E Schellhorn
Oct 1, 1993·Trends in Microbiology·M J Blaser
May 1, 1993·Infection and Immunity·A LeeS Hazell
Jun 1, 1993·Infection and Immunity·C A LingwoodM Huesca

❮ Previous
Next ❯

Citations

Jun 9, 2000·Molecular Microbiology·P R JungblutT F Meyer
Aug 2, 2006·Molecular Microbiology·Ge WangRobert J Maier
Oct 1, 2004·The Journal of Biological Chemistry·Ge WangRobert J Maier
Jun 4, 2010·Antioxidants & Redox Signaling·Hitoshi TsugawaToshifumi Hibi
Nov 5, 1999·Annual Review of Microbiology·Z Ge, D E Taylor
Oct 7, 2015·Infection and Immunity·John T LohTimothy L Cover
Oct 4, 2014·World Journal of Gastroenterology : WJG·Taslima T LinaVictor E Reyes
Oct 11, 2011·PloS One·Patrick S HopfCarole Creuzenet
Aug 23, 2005·Folia Microbiologica·J GodocíkováB Polek
Mar 12, 1999·Journal of Bacteriology·M Tichy, W Vermaas
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·J Daniel DubreuilRino Rappuoli
Jun 18, 2002·Infection and Immunity·Dirk BumannPeter R Jungblut
Nov 18, 2004·Journal of Bacteriology·Nina R SalamaStanley Falkow
Jun 17, 2000·Infection and Immunity·J A KimJ E Mayfield
Nov 12, 2002·Journal of Bacteriology·Nicolette de VriesJohannes G Kusters
Jul 10, 2004·Clinical and Diagnostic Laboratory Immunology·Abdelfattah M AttallahMohamed Abdel-Wahab
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·Nobuyuki SuzukiTakashi Shimoyama
May 19, 2000·Methods : a Companion to Methods in Enzymology·A Ljungh
Dec 1, 2006·Journal of Clinical Microbiology·Omar A OyarzabalSteffen Backert
Oct 23, 1997·Clinical Microbiology Reviews·B E DunnM J Blaser
Nov 26, 2002·Biochimica Et Biophysica Acta·Laura C ChiricaSven Lindskog
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·A MaraisF Mégraud
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·P DoigT J Trust

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.