Cloning and identification of a novel tyrosinase and its overexpression in Streptomyces kathirae SC-1 for enhancing melanin production

FEMS Microbiology Letters
Jing GuoShang-Tian Yang

Abstract

A 30-kDa novel tyrosinase was purified to homogeneity. The Km for L-Dopa and L-tyrosine were determined as 0.42 and 0.25 mM. The 1231 bp (base pair) melC gene and its 167 bp promoter Pskmel were obtained by thermal asymmetric interlaced polymerase chain reaction based on the amino acids fragment obtained from MS results of the purified enzyme. The protein sequence of tyrosinase shows maximum identity (84%) to tyrosinase from Streptomyces galbus. The melC was introduced into S. kathirae. The melanin production and the transcriptional level of melC in recombinant S. kathirae [pIJPskmelmelC] were about 2.1-fold and 2-fold higher than the wild-type strain, respectively. The melanin concentration was maximized at 28.8 g L(-1).

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Citations

Mar 5, 2018·Applied Microbiology and Biotechnology·Jelena SpasicJasmina Nikodinovic-Runic
Dec 8, 2019·Applied Microbiology and Biotechnology·María Elisa PavanM Julia Pettinari
Nov 12, 2019·Frontiers in Bioengineering and Biotechnology·Luz María MartínezGuillermo Gosset
Feb 12, 2021·International Journal of Biological Macromolecules·Fenghua WangYihan Liu
May 1, 2021·Microorganisms·Macarena LarroudeTristan Rossignol
Nov 25, 2021·World Journal of Microbiology & Biotechnology·Mayowa Agunbiade, Marilize Le Roes-Hill

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