Cloning and identification of two novel splice variants of human PD-L2

Acta Biochimica Et Biophysica Sinica
Xianhui HeYao-Ying Zeng

Abstract

PD-L2, a newly identified member of B7 family, plays a role in down-regulating T cell responses. The common PD-L2 mRNA (type I) is the splicing product containing all 6 exons. We report here the identification of two human PD-L2 splice variants in activated leukocytes. One splice variant (type II) is generated through splicing out exon 3 encoding Ig constant-like domain; it retains all other regions without a frame shift. The other variant (type III) is created by splicing out exon 3 to an alternative acceptor site 5 bp downstream of the canonical acceptor site, leading to a frame shift. Consequently, the translated protein should be a soluble form. Furthermore, type I isoform is expressed on the plasma surface whereas type II isoform showed a pattern of intracellular membrane distribution in the transiently transfected K562 cells. In addition, the expression patterns of PD-L2 splice variants are variable in different individuals and distinct cellular status. These results suggest that PD-L2 expression may be controlled by posttranscriptional regulation through alternative splicing, and modulation of PD-L2 isoform expression may influence the outcome of immune response.

Citations

Aug 19, 2006·Immunological Investigations·Dallas B Flies, Lieping Chen
Jan 5, 2008·Annual Review of Immunology·Mary E KeirArlene H Sharpe
Jan 16, 2019·Current Urology Reports·Caroline E Nunes-XavierJosé I López
Nov 5, 2019·Frontiers in Oncology·Oliviero MarinelliMassimo Nabissi
Nov 30, 2018·Journal for Immunotherapy of Cancer·Daqian GuXiang Xu
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Apr 7, 2007·Journal of Immunotherapy·Dallas B Flies, Lieping Chen
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Kaijian ZhouGuoxin Liang

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