PMID: 9427508Jan 14, 1998Paper

Cloning and in situ hybridization analysis of the expression of polysialyltransferase mRNA in the developing and adult rat brain

Brain Research. Molecular Brain Research
G K WoodLalit K Srivastava

Abstract

Polysialyltransferase (PST) is an enzyme that catalyzes the addition of polysialic acid (PSA), a homopolymer of alpha-2,8-linked sialic acid residues, onto neural cell adhesion molecule (NCAM). The expression of PSA-NCAM in the brain is developmentally regulated and is of critical importance; however, the temporal and spatial developmental expression of brain PST, a potential key player in the control of PSA-NCAM levels, remains unclear. In the present study, we have cloned the coding region of rat PST cDNA by reverse transcription-polymerase chain reaction, using primers based on the hamster PST-1 cDNA sequence. A 39-mer oligonucleotide complementary to rat PST cDNA was synthesized to investigate the distribution of its mRNA in the developing and adult rat brain by Northern blot and in situ hybridization. In the embryonic rat brain, PST mRNA was detected abundantly throughout the neuroepithelia of most brain regions. At post-natal days 1 and 14, PST was detected throughout the neocortex, in the pyramidal cells (PC) of the hippocampus proper, the granule cell layer (GCL) of the dentate gyrus, the anterior ventral nucleus of the thalamus (AVNT) and the GCL and external germinal layer of the cerebellum. Finally, from PD21 until a...Continue Reading

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Citations

Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G S Crary, J H Albrecht
Nov 18, 1998·Current Opinion in Structural Biology·M MühlenhoffR Gerardy-Schahn
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