PMID: 9662711Jul 15, 1998Paper

Cloning and in vitro expression of TPK3, a Toxoplasma gondii homologue of shaggy/glycogen synthase kinase-3 kinases

Molecular and Biochemical Parasitology
C L QinK Kim

Abstract

As an initial effort to dissect the signaling pathways responsible for pathogenesis of Toxoplasma gondii infection, we report the cloning and in vitro functional studies of TPK3 (Toxoplasma protein kinase-3), a homologue of shaggy/glycogen synthase kinase-3 (GSK-3) kinases. The shaggy/GSK-3 family of kinases are highly conserved protein kinases that play important roles in cell fate determination, nuclear signaling and hormonal regulation. The TPK3 gene was isolated by RT-PCR with degenerate primers corresponding to highly conserved regions of serine/threonine protein kinases. The complete sequences of genomic and cDNA clones indicated the open reading frame, 1185 bp in size, is interrupted by five introns. The predicted protein sequence of TPK3 shows 54% identity to shaggy/GSK-3 over the catalytic domains. Southern analysis revealed TPK3 is a single copy locus in the Toxoplasma genome. Antisera to other GSK-3 proteins from other species recognized GST-TPK3 and a protein of the predicted size in parasites lysates. In vitro kinase assays with GST-TPK3 indicated that TPK3 autophosphorylates and phosphorylates protein phosphatase inhibitor-2 (I-2), a specific substrate of GSK-3 kinase.

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Citations

Feb 26, 2011·Parasitology·Dmitry I OsolodkinNikolay S Zefirov
Jul 19, 2002·Molecular Microbiology·Farzana KhanKami Kim
Dec 19, 2000·Infection and Immunity·L A KirkmanK Kim
Apr 14, 2000·Frontiers in Bioscience : a Journal and Virtual Library·L M Weiss, K Kim
Aug 23, 2012·PLoS Pathogens·Anita A KoshyJohn C Boothroyd
May 19, 2004·Trends in Parasitology·John EllisDavid Morrison
Sep 21, 2001·The Journal of Biological Chemistry·S A MillerK Kim
Jul 12, 2019·Parasite : Journal De La Société Française De Parasitologie·Md Khalesur Rahman, Myungjo You
Feb 8, 2002·European Journal of Cell Biology·S H MatthiesenB H Satir

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