Cloning and mapping of cat (Felis catus) immunoglobulin and T-cell receptor genes

Immunogenetics
K W ChoA Hasegawa

Abstract

Molecular cloning and chromosomal mapping of the cat immunoglobulin (Ig) and T-cell receptor (TcR) genes were carried out to provide basic information for genetic analysis of immunologic diseases including leukemias and lymphomas in cats. We cloned two Ig constant genes, IGHM and IGHG and three TcR constant genes, TRAC, TRGC, and TRDC, by polymerase chain reaction (PCR) amplification of cDNA from cat peripheral blood mononuclear cells. For chromosomal mapping of the Ig and TcR loci including the IGK, IGL, and TRB on the cat genome, we performed PCR screening of DNAs from 37 cat x rodent somatic cell hybrids by using specific primers for the given genes. Consequently, three loci for IGH, TRA, and TRD, and two loci for TRB and TRG were found to be syntenic and assigned to cat chromosomes (FCA) B3 and A2, respectively. Further, IGK and IGL loci were mapped on FCA A3 and D3, respectively. These findings support the notion that the genetic linkages between the Ig and TcR genes are extensively conserved between humans and cats.

Citations

Feb 11, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K W ChoH Satoh
Mar 4, 2000·Veterinary Immunology and Immunopathology·T H KanaiT Nakamura
May 6, 2008·Veterinary Immunology and Immunopathology·A Th A WeissM Reinacher
Nov 16, 2004·Veterinary Immunology and Immunopathology·Yasuhito FujinoHajime Tsujimoto
Nov 24, 2005·Developmental and Comparative Immunology·Zuly E ParraRobert D Miller
Dec 29, 2012·Journal of Veterinary Science·Alexander Th A WeissAchim D Gruber

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