Cloning and molecular characterisation of resuscitation promoting factor-like gene from Mycobacterium avium subspecies avium

Indian Journal of Medical Microbiology
R Kavitha, R Verma

Abstract

Resuscitation promoting factor (Rpf)-like gene of Mycobacterium avium subspecies paratuberculosis has been known to stimulate the growth of mycobacteria and enhances the recovery of replicating cells from non-replicating phases. The objective of the study was to produce recombinant rpf-like protein of M. avium subspecies avium protein for purification and physico-chemical characterisation. The identified rpf gene of M. avium subspecies avium was cloned, subcloned, sequenced and expressed in Escherichia coli expression system for the production of the recombinant protein. The expressed recombinant Rpf protein was confirmed by Western blot and the extract was purified to yield a pure recombinant protein. An rpf-like gene of 675 bp size in the M. avium subspecies avium was identified. This gene was expressed and the recombinant Rpf weighed 65 kDa as confirmed by Western blot. The M. avium recombinant Rpf protein was extracted under denatured conditions and purified yielding a recombinant protein with >90% purity. Identification, cloning, sequencing and expression of a rpf-like gene from M. avium suggest that RpfA is present in this species also, which might be involved in reactivation phenomenon in this high-risk pathogen.

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Dec 1, 1995·Computer Applications in the Biosciences : CABIOS·C Geourjon, G Deléage
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·G V MukamolovaD B Kell
Jun 14, 2000·Current Opinion in Microbiology·D B Kell, M Young
Nov 2, 2002·Molecular Microbiology·Galina V MukamolovaMichael Young
Nov 2, 2002·Molecular Microbiology·Galina V MukamolovaMichael Young
Aug 9, 2003·Tuberculosis·Wenming ZhuThomas M Shinnick
Feb 22, 2005·Nature Structural & Molecular Biology·Martin Cohen-GonsaudNicholas H Keep
Apr 18, 2006·Biochemistry. Biokhimii︠a︡·M V TelkovA S Kaprelyants
May 6, 2006·Trends in Microbiology·Nicholas H KeepBrian Henderson
Jul 1, 2008·Expert Review of Anti-infective Therapy·Marcelo Corti, Domingo Palmero
Feb 15, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zhenhua YangAase B Andersen

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