Cloning and molecular characterization of a functional flavonoid 3'-hydroxylase gene from Brassica napus

Journal of Plant Physiology
Ben-Bo XuYou-Rong Chai

Abstract

A flavonoid 3'-hydroxylase (F3'H) gene, denoted BnF3'H-1, was cloned from oilseed rape (Brassica napus). The gene of 3038 base pairs (bp) contains 3 introns. The complementary DNA (cDNA) consists of 1820bp and has an open reading frame of 1536bp encoding a polypeptide of 511 amino acids with a molecular weight of 56.62kDa and an isoelectric point of 7.08. BnF3'H-1 shows high homology to known F3'H genes, especially F3'H from Arabidopsis thaliana. Untranslated regions (UTRs) may play important roles in regulating the expression of BnF3'H-1. Besides containing a Kozak sequence, the first 77-bp region is C-rich but G-poor, and the 26-bp 5'-UTR contains 3 sites of ACCACT-like sequences. Alternative polyadenylation in the 3'-UTR is adopted by this gene to generate heterogeneous transcripts. Conserved domain search and motif characterization identified BnF3'H-1 as a cytochrome P450. All F3'H-featured motifs, VVVAAS, GGEK and VDVKG, are unchanged in BnF3'H-1. The N-terminal signal peptide/anchor and 3 transmembrane helices were predicted in BnF3'H-1, and its subcellular localization is most probably at the endoplasmic reticulum. Since 16 phosphorylation sites could be predicted, phosphorylation may be a necessary post-translational mo...Continue Reading

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Citations

Feb 22, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Liezhao LiuRod J Snowdon
Oct 5, 2015·Plant Physiology and Biochemistry : PPB·Run-Ze SunJun Wang
Aug 13, 2015·Journal of Agricultural and Food Chemistry·Tien Thanh VuHojoung Lee
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Nov 25, 2016·Frontiers in Plant Science·Cunmin QuJia-Na Li

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