Cloning and molecular characterization of TaERF6 , a gene encoding a bread wheat ethylene response factor

Molecular Biology Research Communications
Shahrzad Haghir, Abbas Alemzadeh

Abstract

Ethylene response factor proteins are important for regulating gene expression under different stresses. Different isoforms for ERF have previously isolated from bread wheat (Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5. We isolated, cloned and molecular characterized a novel one based on TdERF1, an isoform in durum wheat (Triticum turgidum L.) and called TaERF6. Its cDNA was synthesized, sequenced and compared with genomic sequence to figure out intron and exon regions and determine coding sequence region. The length of TdERF1 gene was 1939 bp and cDNA was 1065 bp including two exons, the first one 259 bp and the second one 806 bp separated by a 874 bp intron with a 111 bp 5'-UTR (untranslated region) and 401 bp 3'-UTR. TaERF6 encodes a 353 amino acids protein with nearly 99% identity to TdERF1. Hydrophobic cluster analysis revealed an N-terminal hydrophobic domain contains a highly conserved motif with the consensus sequence of M [C/L/Y] [G/R] [G/R/P] [A/G/V/L/R] [I/L/R/S/P/Q] [L/I/R/H] and hydrophobic clusters in AP2/ERF domain of which tends to form -sheet. Three monopartite nuclear localization signals also identified in TaERF6 that play important role in getting back into the nucleus. The resu...Continue Reading

Datasets Mentioned

BETA
KJ689809.1
KJ689810.1
KJ689811.1
KJ689812.1
KJ689813.1
AK335399.1
AK452621.1
AY781361.1
AF542184.1
AK332004.1

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

TrEMBL
ExPASy
Clustal Omega
RPS
ClustalW
Expert Protein Analysis System ) ExPASy
HCA
PredictNLS
MEGA6
Kalign

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