Cloning and molecular dissection of the 8.8 kb pig uroplakin II promoter using transgenic mice and RT4 cells

Journal of Cellular Biochemistry
Deug-Nam KwonJin-Hoi Kim

Abstract

Uroplakin II (UPII) gene expression is highly tissue and cell specific, with mRNA present in the suprabasal cell layers of the bladder and urethra. Previous reports described the mouse UPII (mUPII) promoter as primarily urothelium selective. However, ectopic expression of a transgene under the 3.6 kb mUPII promoter was also detected in brain, kidney, and testis in some transgenic mouse lines. Here, we have cloned an 8.8 kb pig UPII (pUPII) promoter region and investigated which cells within the bladder and urethra express a transgene consisting of the pUPII promoter fused to human erythropoietin (hEPO) or a luciferase gene. pUPII-luciferase expression vectors with various deletions of the promoter region were introduced into mouse fibroblast (NIH3T3), Chinese hamster ovary (CHO), and human bladder transitional carcinoma (RT4). A 2.1 kb pUPII promoter fragment displayed high levels of luciferase activity in transiently transfected RT4 cells, whereas the 8.8 kb pUPII promoter region displayed only low levels of activity. The pUPII-hEPO expression vector was injected into the pronucleus of zygotes to make transgenic mice. To elucidate the in vivo molecular mechanisms controlling the tissue- and cell-specific expression of the pUPI...Continue Reading

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Citations

Mar 29, 2012·Journal of Inherited Metabolic Disease·Yonglun LuoCharlotte Brandt Sørensen
Dec 25, 2008·The Journal of Reproduction and Development·Seong-Keun ChoJin-Hoi Kim
Mar 6, 2015·The Journal of Reproduction and Development·Byung-Soo ChangJin-Hoi Kim
Feb 10, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jae-Hwan KimJin-Hoi Kim
Sep 13, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Seong-Keun ChoJin-Hoi Kim
Jun 11, 2011·Biochemical and Biophysical Research Communications·Deug-Nam KwonJin-Hoi Kim

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