PMID: 9632602Jun 25, 1998Paper

Cloning and mutagenesis of a serotype-specific DNA region involved in encapsulation and virulence of Actinobacillus pleuropneumoniae serotype 5a: concomitant expression of serotype 5a and 1 capsular polysaccharides in recombinant A. pleuropneumoniae serotype 1

Infection and Immunity
C K WardThomas Inzana

Abstract

A DNA region involved in Actinobacillus pleuropneumoniae serotype 5 capsular polysaccharide (CP) biosynthesis was identified and characterized by using a probe specific for the cpxD gene involved in CP export. The adjacent serotype 5-specific CP biosynthesis region was cloned from a 5.8-kb BamHI fragment and an 8.0-kb EcoRI fragment of strain J45 genomic DNA. DNA sequence analysis demonstrated that this region contained four complete open reading frames, cps5A, cps5B, cps5C, and cps5D. Cps5A, Cps5B, and Cps5C showed low homology with several bacterial glycosyltransferases involved in the biosynthesis of lipopolysaccharide or CP. However, Cps5D had high homology with KdsA proteins (3-deoxy-D-manno-2-octulosonic acid 8-phosphate synthetase) from other gram-negative bacteria. The G+C content of cps5ABC was substantially lower (28%) than that of cps5D and the rest of the A. pleuropneumoniae chromosome (42%). A 2.1-kb deletion spanning the cloned cps5ABC open reading frames was constructed and transferred into the J45 chromosome by homologous recombination with a kanamycin resistance cassette to produce mutant J45-100. Multiplex PCR confirmed the deletion in this region of J45-100 DNA. J45-100 did not produce intracellular or extrac...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M FroschT F Meyer
Sep 1, 1989·Antimicrobial Agents and Chemotherapy·P J WillsonJ K Setlow
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Dec 30, 1987·European Journal of Biochemistry·E AltmanM B Perry
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jan 1, 1965·Annual Review of Microbiology·G N Cohen
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Feb 1, 1984·Journal of Bacteriology·R P SilverW Aaronson
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jan 1, 1993·Advances in Microbial Physiology·C Whitfield, M A Valvano
Sep 1, 1993·Journal of Bacteriology·R I TasconJ A Vazquez-Boland
Jun 10, 1998·Journal of Clinical Microbiology·T M LoT J Inzana

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Citations

Sep 26, 2006·FEMS Immunology and Medical Microbiology·Trevor K Wagner, Martha H Mulks
Jul 30, 2020·Scientific Reports·Hugo F AzurmendiWillie F Vann
May 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Olga G OvchinnikovaChris Whitfield
Nov 16, 2001·Animal Health Research Reviews·J D DubreuilM Gottschalk
Aug 26, 2009·Infection and Immunity·Sargurunathan SubashchandraboseMartha H Mulks
Aug 31, 2010·Journal of Bacteriology·Zhuofei XuRui Zhou
Apr 16, 2002·Infection and Immunity·Linda J McKerral, Reggie Y C Lo
May 23, 2003·Infection and Immunity·Aloka B BandaraThomas J Inzana
May 20, 2008·The Veterinary Record·L ZhouP R Langford
Sep 23, 2000·Journal of Biotechnology·J D BoyceB Adler
Sep 15, 2004·Journal of Clinical Microbiology·Jennifer A SchuchertS Jessing

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