Cloning and nucleotide sequence of a flagellin encoding genetic locus from Xenorhabdus nematophilus: phase variation leads to differential transcription of two flagellar genes (fliCD)

Gene
A GivaudanN Boemare

Abstract

The insect-pathogenic bacterium Xenorhabdus undergoes spontaneous phase variation involving a large number of phenotypes. Our previous study indicated that phase I variants were motile, whereas phase II variants of X. nematophilus F1 were nonflagellated cells which did not synthesize flagellin [Givaudan A., Baghdiguian, S., Lanois, A. and Boemare, N. (1995) Appl. Environ. Microbiol. 61, 1408-1413]. In order to approach the study of the flagellar switching, a locus containing two ORFs from X. nematophilus F1 (phase I) was identified by using functional complementation of flagellin-negative E. coli. The sequence analysis revealed that the first ORF corresponds to the fliC gene coding for flagellin, and showed a high degree of homology between the N-terminal and C-terminal of Xenorhabdus FliC and flagellins from other bacteria. The second identified ORF in the opposite orientation encodes a homologue of the enterobacterial hook-associated protein 2, FliD. Both Xenorhabdus fliCD genes were required for the entire restoration of E. coli motility. A sequence highly homologous to the sigma 28 consensus promoter was identified upstream from the coding sequences from both genes. The structure of the fliC gene and its surrounding region ...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Sep 1, 1991·Applied and Environmental Microbiology·M C Leclerc, N E Boemare
Feb 1, 1990·Journal of Bacteriology·K KutsukakeT Iino
Oct 1, 1989·Molecular Microbiology·G FrankelB A Stocker
Apr 1, 1989·Applied and Environmental Microbiology·J XuR E Hurlbert
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jun 1, 1982·Journal of Clinical Microbiology·D J BrennerA G Steigerwalt
Jun 1, 1994·Journal of Bacteriology·M E PowerT J Trust
Jan 1, 1995·Applied and Environmental Microbiology·G B LeismanS A Forst
Nov 1, 1993·Molecular Microbiology·K Dybvig
Apr 1, 1995·Applied and Environmental Microbiology·A GivaudanN Boemare
Jul 1, 1995·Applied and Environmental Microbiology·N MoureauxN Boemare

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Citations

Jul 30, 2008·Antonie van Leeuwenhoek·Florence Wisniewski-Dyé, Ludovic Vial
Aug 5, 2000·Applied and Environmental Microbiology·R H Ffrench-ConstantF R Blattner
Jan 1, 1997·Annual Review of Microbiology·S ForstE Stackebrandt
Dec 14, 2005·Applied and Environmental Microbiology·A VolgyiS Forst

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