PMID: 2509433Nov 1, 1989Paper

Cloning and nucleotide sequence of braC, the structural gene for the leucine-, isoleucine-, and valine-binding protein of Pseudomonas aeruginosa PAO

Journal of Bacteriology
T Hoshino, K Kose

Abstract

The gene for the leucine-, isoleucine-, and valine-binding protein (LIVAT-BP) in Pseudomonas aeruginosa PAO was isolated, and its nucleotide sequence was determined. The gene consisted of 1,119 nucleotides specifying a protein of 373 amino acid residues. Determination of the N-terminal amino acid sequence of the LIVAT-BP purified from P. aeruginosa shock fluid suggested that the N-terminal 26 residues of the gene product are cleaved off posttranslationally, showing the characteristic features of procaryotic signal peptides. The amino acid composition of the mature product predicted from the nucleotide sequence was in good agreement with that of the purified LIVAT-BP. The plasmid carrying the LIVAT-BP gene restored the activity of the high-affinity branched-chain amino acid transport system (the leucine, isoleucine, valine [LIV-I] transport system) in the braC310 mutant of P. aeruginosa, confirming that braC is the structural gene for LIVAT-BP. The mutant LIVAT-BP lacking a 16-amino-acid peptide in the middle was found to be functional in the LIV-I transport system. LIVAT-BP showed extensive homology (51% identical) to the LIV- and leucine-specific-binding proteins of Escherichia coli K-12, which are coded for by the livJ and li...Continue Reading

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Citations

Dec 16, 1998·Nature Biotechnology·N UekiM Muramatsu
Apr 4, 2002·Molecular Microbiology·Ana ValladaresEnrique Flores
Aug 1, 1990·Journal of Bioenergetics and Biomembranes·C F HigginsM P Gallagher
Feb 28, 2004·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Jonathan E Cohen, R Douglas Fields
Dec 7, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Victoria Coutinho, Thomas Knöpfel
Jun 26, 2001·Research in Microbiology·A H Hosie, P S Poole

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