Cloning and partial characterization of Entamoeba histolytica PTPases

Biochemical and Biophysical Research Communications
Sara Elisa Herrera-RodríguezJosé Luis Rosales-Encina

Abstract

Reversible protein tyrosine phosphorylation is an essential signal transduction mechanism that regulates cell growth, differentiation, mobility, metabolism, and survival. Two genes coding for protein tyrosine phophatases, designed EhPTPA and EhPTPB, were cloned from Entamoeba histolytica. EhPTPA and EhPTPB proteins showed amino acid sequence identity of 37%, both EhPTPases showed similarity with Dictyostelium discoideum and vertebrate trasmembranal PTPases. mRNA levels of EhPTPA gene are up-regulated in trophozoites recovered after 96h of liver abscess development in the hamster model. EhPTPA protein expressed as a glutathione S-transferase fusion protein (GST::EhPTPA) showed enzymatic activity with p-nitrophenylphosphate as a substrate and was inhibited by PTPase inhibitors vanadate and molybdate. GST::EhPTPA protein selectively dephosphorylates a 130kDa phosphotyrosine-containing protein in trophozoite cell lysates. EhPTPA gene codifies for a 43kDa native protein. Up-regulation of EhPTPA expression suggests that EhPTPA may play an important role in the adaptive response of trophozoites during amoebic liver abscess development.

References

Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S DiamondC C Cunnick
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 14, 1992·Biochimica Et Biophysica Acta·D L Brautigan
Oct 4, 1991·Cell·W G Dunphy, A Kumagai
Dec 11, 1991·Nucleic Acids Research·S Henikoff, J G Henikoff
Jun 1, 1989·Annals of Tropical Medicine and Parasitology·S K KatiyarP Sagar
Apr 1, 1994·Trends in Biochemical Sciences·L J Mauro, J E Dixon
Jun 1, 1994·Molecular and Cellular Biology·R H DiamondR Taub
Apr 26, 2000·International Journal for Parasitology·M M Aguirre-GarcíaP Talamás-Rohana
Oct 5, 2001·Molecular and Cellular Biology·J N AndersenN P Møller
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Zhong-Yin Zhang
May 16, 2002·Molecular Microbiology·Iris BruchhausEgbert Tannich
Mar 28, 2003·Lancet·Samuel L Stanley
Jun 18, 2003·Experimental Parasitology·Donaciano Flores-RoblesPatricia Talamás-Rohana
Jul 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ravid SassonAbraham Amsterdam
Jan 14, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jannik N AndersenNiels Peter H Møller
Mar 1, 1994·Trends in Cell Biology·S M Brady-Kalnay, N K Tonks
Jun 23, 2004·Experimental Parasitology·Bibiana Chávez-MunguíaAdolfo Martínez-Palomo
Apr 9, 2005·The Journal of Endocrinology·Andrew W Stoker

❮ Previous
Next ❯

Citations

Oct 4, 2015·Experimental Parasitology·Ana Lilia Ramírez-TapiaJosé Luis Rosales-Encina
Nov 23, 2006·Experimental Parasitology·Ana Acacia de Sá PinheiroJosé Roberto Meyer-Fernandes
Feb 13, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Terrence J RavineJames Jenkins
Jul 27, 2021·Parasitology Research·Jenny Nancy Gómez-SandovalM Magdalena Aguirre-García

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.