PMID: 2507177Jul 1, 1989Paper

Cloning and physical characterization of linked lysine genes (lys4, lys15) of Saccharomyces cerevisiae

Current Genetics
L WangJ K Bhattacharjee

Abstract

The plasmid pSC4 which carries a 7.8 kb yeast DNA insert at the BamHI site of the Vector YEp13, complemented simultaneously MO-59-13c lys4, LU75 lys15 and LU32 lys4lys15 (double) mutations of Saccharomyces cerevisiae. The 1.9 kb BamHI-XbaI DNA insert of the subclone pSO51 complemented the LU75 lys15 mutation. The 2.8 kb Xhol-XhoI DNA insert of the pSO52 subclone, like pSC4, complemented all three mutations. The 1.9 kb BamHI-XbaI DNA and the 2.8 kb Xhol-XhoI DNA were 100 bp apart in the pSC4 DNA insert and exhibited no homology with each other upon Southern hybridization. The 1.9 kb BamHI-XbaI DNA insert exhibited homology with the pSC4 and pSO51 DNA as well as the genomic DNA of MO-59-13c lys4, LU75 lys15, LU32 lys4lys15, and RC1 (LYS) when digested with appropriate restriction enzymes. The 2.8 kb XhoI-XhoI DNA insert exhibited homology with the pSC4 and pSO52 DNA as well as MO-59-13c lys4, LU75 lys15, LU32 lys4lys15, and RC1 (LYS) genomic DNA, when digested with XhoI enzyme. The 2.8 kb DNA probe also hybridized with ply(A)+ RNA from RC1 and lys4+ transformant but not that from MO-59-13c lys4 mutant.

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Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·T Kosuge, T Hoshino
Dec 30, 1998·FEMS Microbiology Letters·T Kosuge, T Hoshino
Jun 30, 2016·Biochemical and Biophysical Research Communications·Eunsoo DoWon Hee Jung
Sep 14, 2006·European Addiction Research·Jacek MoskalewiczYu Ye
Jan 13, 2004·Veterinary Pathology·F C OriggiE R Jacobson

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