PMID: 2111143Apr 1, 1990Paper

Cloning and prokaryotic expression of a biologically active human placental aldose reductase

DNA and Cell Biology
U GrundmannE Amann

Abstract

cDNA clones coding for human aldose reductase (AR) were isolated by antibody screening of a placental lambda gt11 cDNA library. The cDNA comprises the entire coding region and has a total length of 1,394 bp. The sequence deduced from the open reading frame encodes a protein of 316 amino acids and its amino acid composition is identical to the placental protein 9 (PP9), whose isolation and characterization were described by Bohn et al. (1982). The amino acid sequence of the placental human AR shows high homology to the rat AR; both proteins belong to the same protein superfamily as human liver AR, frog lens rho-crystallin, and bovine lung prostaglandin F synthase. Northern blot hybridization analysis revealed a size for the AR mRNA of approximately 1,500 bases. In addition to the full-length cDNA, one lambda gt11 clone was isolated which carries a putative intron of 597 bp at nucleotide position 754, corresponding to amino acid position 247. Expression of the AR cDNA in Escherichia coli resulted in the synthesis of a protein with a molecular weight of approximately 35 kD which can be immunoprecipitated specifically with antiserum raised against PP9. Despite the absence of a typical signal sequence, the human aldose reductase is ...Continue Reading

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Citations

Jan 1, 1991·Archives of Gynecology and Obstetrics·H BohnU Grundmann
Mar 1, 1996·Journal of Diabetes and Its Complications·A PatelA G Demaine
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·B Karuna SreeA R. Reddy
Jan 1, 1995·European Journal of Pediatrics·G T Berry
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·A Bhatnagar, S K Srivastava
Jul 14, 1995·The Journal of Biological Chemistry·T J Kubiseski, T G Flynn
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuying C HwangRavichandran Ramasamy

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