PMID: 11931040Apr 5, 2002Paper

Cloning and sequence analysis of a cDNA encoding Pso o II, a mite group II allergen of the sheep scab mite (Acari: Psoroptidae)

Journal of Medical Entomology
Kevin B TemeyerJohn H Pruett

Abstract

Psoroptes ovis (Hering), the sheep scab mite, is responsible for psoroptic scabies of cattle and sheep. Reverse translation of 30 N-terminal amino acids of the major P. ovis allergen, previously chosen as a candidate immunogen and identified as a 16 kDa protein yielded a degenerate sequence used to design oligodeoxynucleotide polymerase chain reaction (PCR) primers. Use of the PCR primers with a P. ovis cDNA library succeeded in amplification of a 90 bp cDNA gene fragment that was cloned, sequenced, and used to select unique sequencing/PCR primers. Primer walking generated overlapping subclones which yielded the 588 nucleotide consensus sequence of the cDNA encoding the 143 amino acid P. ovis allergen precursor. Nucleotide and translated sequences of the cDNA were compared with sequences in GenBank and found to be homologous to mite group II allergens Lep d II (formerly Lep d I) of Lepidoglyphus destructor Schrank, DerfII of Dermatophagoides farinae Hughes, Der p II of Derrmatophagoides pteronyssinus (Trouessart), Tyr p II of Tyrophagus putrescentiae (Schrank), Eur m II of Euroglyphus maynei (Cooreman) and Gly d II of Glycophagus domesticus (De Geer). The mature P. ovis allergen is composed of 126 amino acids with a calculated ...Continue Reading

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Citations

Mar 20, 2012·Cell and Tissue Research·Deborah C HoltKatja Fischer
Aug 11, 2005·Current Allergy and Asthma Reports·Enrique Fernández-Caldas, Víctor Iraola Calvo
Apr 30, 2013·Veterinary Parasitology·J L Fitzpatrick
Feb 6, 2007·Parasitology·P Willadsen
Apr 25, 2012·Veterinary Parasitology·Bertrand Jacques Losson
Oct 4, 2011·Molecular and Cellular Probes·Francesca G NunnJohn F Huntley
Oct 20, 2009·Veterinary Immunology and Immunopathology·Richard ShawRobert Bischof
May 13, 2008·Veterinary Parasitology·Kevin B TemeyerLane D Foil
Mar 17, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Kevin B Temeyer, Andrew C Chen
Dec 18, 2019·Parasites & Vectors·Ran HeGuang-You Yang

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