PMID: 3756168Oct 16, 1986Paper

Cloning and sequence analysis of cDNA for mouse prolactin

Biochimica Et Biophysica Acta
T HarigayaK Hoshino

Abstract

The present study was undertaken to find out whether or not sexual dimorphism in biological activities and amino acid compositions of mouse prolactin might be due to heterogeneity in mRNA for mouse prolactin Cloned cDNAs for mouse prolactin were first isolated from a mouse pituitary cDNA library by hybridization with a rat prolactin cDNA. Then, one clone of about 140 positive clones obtained from 2000 transformants was subjected to nucleotide sequence analysis and verified to contain a nearly full length of cDNA sequence coding for mouse prolactin precursor. The deduced complete amino acid sequence indicates that the precursor molecule consists of 31 amino acids as the signal peptide and 197 amino acids of prolactin, in which two amino acids were found to be different from the amino acid sequence previously published elsewhere. S1 nuclease mapping analysis using male and female pituitary RNAs indicates that mouse preprolactin is encoded by two mRNAs in both sexes. The two mRNAs differ from each other based upon the deletion of three nucleotides in the coding region for the signal peptide determined by the nucleotide sequence analysis in other cDNA clones. In the present study, no sexual difference was revealed in murine prolact...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A J Berk, P A Sharp
Jan 1, 1979·Methods in Enzymology·D A Morrison
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Sep 16, 1976·Nature·N J Proudfoot, G G Brownlee
Apr 1, 1985·Endocrinology·T W Strickland, J G Pierce
Aug 15, 1985·Biochemical and Biophysical Research Communications·H HashimotoK Hoshino
Oct 15, 1981·Biochemical and Biophysical Research Communications·W L TaylorJ E Dixon
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Feb 1, 1982·Molecular and Cellular Biology·H Okayama, P Berg
Jan 1, 1983·Endocrine Reviews·W L Miller, N L Eberhardt
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H OhkuboS Nakanishi
Jan 16, 1984·European Journal of Biochemistry·K KohmotoF Sakiyama
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Jun 1, 1980·Gene·D Hanahan, M Meselson
Aug 29, 1980·Biochemical and Biophysical Research Communications·I Mittra

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Citations

Feb 18, 2004·The Journal of Reproduction and Development·Minoru KoizumiToshio Harigaya
Mar 9, 2002·General and Comparative Endocrinology·Lori A Manzon
Sep 26, 2013·Molecular and Cellular Endocrinology·Y Sangeeta Devi, Julia Halperin
May 1, 1988·Journal of Medical Genetics·L C Tsui

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