PMID: 8595669Feb 1, 1996Paper

Cloning and sequence analysis of the invertase gene INV 1 from the yeast Pichia anomala

Current Genetics
J A PérezT Ruiz

Abstract

A genomic library from the yeast Pichia anomala has been constructed and employed to clone the gene encoding the sucrose-hydrolysing enzyme invertase by complementation of a sucrose non-fermenting mutant of Saccharomyces cerevisiae. The cloned gene, INV1, was sequenced and found to encode a polypeptide of 550 amino acids which contained a 22 amino-acid signal sequence and ten potential glycosylation sites. The amino-acid sequence shows significant identity with other yeast invertases and also with Kluyveromyces marxianus inulinase, a yeast beta-fructofuranosidase which has a different substrate specificity. The nucleotide sequences of the 5' and 3' non-coding regions were found to contain several consensus motifs probably involved in the initiation and termination of gene transcription.

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Citations

Aug 15, 2001·Research in Microbiology·G I NaumovJ Schnürer
Dec 18, 2008·Applied and Environmental Microbiology·Dolores LindeMaría Fernández-Lobato
Jan 21, 2006·FEMS Yeast Research·Volkmar PassothJohan Schnürer
Jan 18, 2006·Letters in Applied Microbiology·S TürkelN Kayakent
Jul 29, 2004·The Journal of Biological Chemistry·Raúl CondeGermán Larriba

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