Cloning and sequence analysis of the plasmid-borne genes encoding the Eco29kI restriction and modification enzymes

Gene
M V ZakharovaA S Solonin

Abstract

The Eco29kI restriction-modification system (RMS2) has been found to be localized on the plasmid pECO29 occurring naturally in the Escherichia coli strain 29k (Pertzev, A.V., Ruban, N.M., Zakharova, M.V., Beletskaya, I.V., Petrov, S.I., Kravetz, A.N., Solonin, A.S., 1992. Eco29kI, a novel plasmid encoded restriction endonuclease from Escherichia coli. Nucleic Acids Res. 20, 1991). The genes coding for this RMS2, a SacII isoschizomer recognizing the sequence CCGCGG have been cloned in Escherichia coli K802 and sequenced. The DNA sequence predicts the restriction endonuclease (ENase) of 214 amino acids (aa) (24,556 Da) and the DNA-methyltransferase (MTase) of 382 aa (43,007 Da) where the genes are separated by 2 bp and arranged in tandem with eco29kIR preceding eco29kIM. The recombinant plasmid with eco29kIR produces a protein of expected size. MEco29kI contains all the conserved aa sequence motifs characteristic of m5C-MTases. Remarkably, its variable region exhibits a significant similarity to the part of the specific target-recognition domain (TRD) from MBssHII--multispecific m5C-MTase (Schumann, J.J., Walter, J., Willert, J., Wild, C., Koch D., Trautner, T.A., 1996. MBssHII: a multispecific cytosine-C5-DNA-methyltransferase w...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 25, 1992·Nucleic Acids Research·A V PertzevA S Solonin
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
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Jan 1, 1996·Nucleic Acids Research·R J Roberts, D Macelis

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Citations

Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q XuM J Blaser
Dec 20, 2014·Journal of Immunological Methods·Erika G HollandMargaret M Kiss
Jul 3, 2007·Biochimica Et Biophysica Acta·Dmitri NikitinAlexander Solonin
May 29, 2004·Molecular Microbiology·Karin CarlsonAnna-Chey Nyström
Oct 1, 2017·Genome Announcements·Gabriele R M Kleiner-GroteKarl Friehs

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