PMID: 9420030Jan 7, 1998Paper

Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations

Antimicrobial Agents and Chemotherapy
D E TaylorK Hiratsuka

Abstract

In this study, two identical copies of a 23S-5S gene cluster, which are separately situated within the Helicobacter pylori UA802 chromosome, were cloned and sequenced. Comparison of the DNA sequence of the H. pylori 23S rRNA gene with known sequences of other bacterial 23S rRNA genes indicated that the H. pylori UA802 23S rRNA genes are closely related to those of Campylobacter spp. and therefore belong in the proposed Proteobacteria subdivision. The 5'-terminal nucleotide T or A of the 23S rRNA is close to a Pribnow box which could be a -10 region of the transcription promoter for the 23S rRNA gene, suggesting that a posttranscriptional process is likely not involved in the maturation of the H. pylori 23S rRNA. Clinical isolates of H. pylori resistant to clarithromycin were examined by using natural transformation and pulsed-field gel electrophoresis. Cross-resistance to clarithromycin and erythromycin, which was transferred by natural transformation from the Cla(r) Ery(r) donor strain H. pylori E to the Cla(s) Ery(s) recipient strain H. pylori UA802, was associated with an single A-to-G transition mutation at position 2142 of both copies of the 23S rRNA in UA802 Cla(r) Ery(r) mutants. The transformation frequency for Cla(r) a...Continue Reading

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Citations

Feb 22, 2001·Antimicrobial Agents and Chemotherapy·G WangD E Taylor
Jan 31, 2003·Journal of Molecular Recognition : JMR·K T MomynalievA I Archakov
May 4, 2005·FEMS Immunology and Medical Microbiology·Rebecca J GorrellRoy M Robins-Browne
Nov 15, 2005·The Journal of Antimicrobial Chemotherapy·Patrizia PosteraroGiovanni Fadda
Jan 12, 2007·Clinical Pharmacology and Therapeutics·T FurutaA Hishida
Sep 26, 2007·Journal of Medical Microbiology·Karen-Anja ModerBrigitte König
Jun 1, 2010·Memórias do Instituto Oswaldo Cruz·Katarine Antonia dos Santos BarileTereza Cristina de Oliveira Corvelo
Oct 18, 2012·Alimentary Pharmacology & Therapeutics·C Y TayB J Marshall
Dec 1, 2007·Expert Opinion on Medical Diagnostics·Ozlem Yilmaz
Mar 22, 2014·The Journal of Antimicrobial Chemotherapy·Tran Thanh BinhYoshio Yamaoka
Sep 5, 2015·Journal of Medical Microbiology·Teresa FascianaAnna Giammanco
May 10, 2016·Journal of Pediatric Gastroenterology and Nutrition·Carolina A SerranoPaul R Harris
Jan 9, 2019·Frontiers in Microbiology·Aldona BińkowskaGrażyna Gościniak
Nov 5, 1999·Annual Review of Microbiology·Z Ge, D E Taylor
Dec 20, 2000·Antimicrobial Agents and Chemotherapy·B Vester, S Douthwaite
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Carla FontanaCartesio Favalli
Dec 22, 2004·Microbiology and Immunology·Kyoko KanaiYoshichika Arakawa
Sep 1, 2007·Helicobacter·Leonardo Garrido, Hector Toledo
Jul 27, 2010·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Mubarak S Alfaresi, Abida A Elkoush
Sep 16, 2014·Journal of Pediatric Gastroenterology and Nutrition·Midori MituiJason Y Park
Mar 6, 2015·Jundishapur Journal of Microbiology·Omid Tadjrobehkar, Hamid Abdollahi
Aug 17, 2002·Antimicrobial Agents and Chemotherapy·Lai-King NgLouis Bryden
Jan 31, 2002·World Journal of Gastroenterology : WJG·Hua-Xiang XiaNicholas J Talley
Jun 26, 2001·Symposium Series·J G Kusters, E J Kuipers
Sep 12, 2002·Expert Opinion on Therapeutic Targets·Zhongming Ge

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