Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin

Gene
Robin S SimmondsJ R Tagg

Abstract

The nucleotide sequence has been determined for zooA, a gene encoding the bacteriocin-like inhibitory substance zoocin A in Streptococcus zooepidemicus strain 4881. The zooA gene product corresponds to the 285-amino acid (aa) zoocin A pre-peptide from which a leader sequence is cleaved to form the 262-aa biologically active molecule of estimated molecular mass 27,877 Da. Expression of zooA in a Gram-negative host was shown by the extracellular release from Escherichia coli, containing cloned zooA, of a biologically active peptide having an identical range of anti-bacterial activity to that of zoocin A, purified from S. zooepidemicus strain 4881. Data base searches revealed sequences having homologies with known muralytic proteins produced by both Gram-positive and Gram-negative bacteria and indicate a 'mix and match' blending of domain-type structures, the C-terminal putative receptor-recognition region of the molecule being joined by a threonine-proline-rich linker to an N-terminal putative catalytic region having homology with several known endopeptidases, including lysostaphin.

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P Bork, R F Doolittle
May 15, 1989·Biochemical and Biophysical Research Communications·H E HeathG L Sloan
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P A RecseiR P Novick
Oct 5, 1973·European Journal of Biochemistry·O J Iversen, A Grov

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Citations

Apr 4, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yinghua ChenRussell Timkovich
May 3, 2003·Trends in Biotechnology·John R Tagg, Karen P Dierksen
Nov 13, 2003·FEMS Microbiology Letters·M Heath FarrisGary L Sloan
Jul 10, 2008·International Journal of Antimicrobial Agents·M L ChikindasJ R Tagg
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Mar 13, 2012·Applied and Environmental Microbiology·Kari Helene BergLeiv Sigve Håvarstein
May 7, 2003·Applied and Environmental Microbiology·Philip A Wescombe, John R Tagg
May 7, 2003·Applied and Environmental Microbiology·Trine NilsenHelge Holo
Nov 30, 2011·Journal of Bacteriology·Kari Helene BergLeiv Sigve Håvarstein
May 20, 2004·Journal of Bacteriology·John G KennyDouwe van Sinderen
Apr 19, 2010·Pharmaceuticals·Maria do Carmo de Freire BastosMarcus Lívio Varella Coelho
Nov 28, 2012·Proteins·Yinghua ChenRussell Timkovich
Apr 6, 2004·Biochemical and Biophysical Research Communications·Qiaoli LiangRussell Timkovich
Oct 28, 2014·Environmental Microbiology·Jinshui ZhengMing Sun
Oct 1, 2013·Biochemical and Biophysical Research Communications·Yinghua ChenRussell Timkovich
Feb 27, 2007·FEMS Microbiology Letters·Maria AkessonRobin S Simmonds
May 28, 2016·BMC Genomics·Ashok K SharmaVineet K Sharma
Jul 12, 2008·FEMS Microbiology Letters·Ying MaoChengping Lu
Jun 19, 1998·FEMS Microbiology Letters·S A BeatsonR S Simmonds

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