Cloning and sequence of a region of Vibrio cholerae O139 Bengal and its use in PCR-based detection.

Journal of Clinical Microbiology
S FalklindA Weintraub

Abstract

We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region could be used for the specific detection of V. cholerae O139 Bengal, a PCR system was established. The specificity and sensitivity of the PCR were investigated by analyzing 240 strains within the family Vibrionaceae and 178 stains of other gram-negative bacteria. All V. cholerae O139 Bengal strains tested were positive, and none of the 384 control strains were amplified. The sensitivity of the assay was 10(2) CFU/ml.

References

Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·U H StroeherP A Manning
Jan 1, 1994·Clinical and Diagnostic Laboratory Immunology·F QadriM J Albert
Aug 1, 1995·Journal of Clinical Microbiology·G B NairY Takeda
Oct 1, 1994·Journal of Clinical Microbiology·M J Albert
Aug 14, 1993·Lancet·D L Swerdlow, A A Ries
Oct 1, 1993·Journal of Clinical Microbiology·N SeesodM Uhlén
Apr 1, 1996·Journal of Clinical Microbiology·M StarkJ Lundeberg

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