Cloning and sequencing of a cDNA encoding chicken mannan-binding lectin (MBL) and comparison with mammalian analogues

Immunology
S B LaursenJ C Jensenius

Abstract

The serum lectin, mannan-binding lectin (MBL) (also denoted mannan-binding protein or mannose-binding protein, MBP) has been identified in mammals (humans, monkey, cow, rabbit, mouse and rat). Upon binding to carbohydrates on the surface of microorganisms, MBL mediates activation of the complement system, leading to killing of the microorganism. MBL thus exerts a role in the innate immune defence. We have described the isolation and partial characterization of an analogous protein in chicken serum. Oligonucleotides based on the N-terminal sequence of this protein were used in a reverse transcription-polymerase chain reaction (RT-PCR) with chicken liver RNA as template. The PCR product was sequenced and found to encode part of the NH2 terminus of chicken MBL. A perfect match probe was synthesized and used to screen a chicken liver cDNA library. The isolated clones carried a cDNA insert of 1692 bp with an open reading frame of 714 bp encoding a mature protein of 238 amino acids including a signal peptide of five amino acids. The deduced amino acid sequence agrees with those determined by conventional amino acid sequence analysis of the peptides except for four residues. We have compared the deduced primary structure of chicken MB...Continue Reading

References

Dec 1, 1992·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·P GarredA Svejgaard
Jun 29, 1991·Lancet·M SumiyaJ A Summerfield
Sep 15, 1989·The Biochemical Journal·M E TaylorJ A Summerfield
Jan 1, 1988·Archives of Biochemistry and Biophysics·S OkaI Yamashina
Jun 4, 1974·Biochemistry·V GlisinC Byus
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Sep 1, 1983·Journal of Biochemistry·N KawasakiI Yamashina
Jul 31, 1980·Biochemical and Biophysical Research Communications·Y KozutsumiI Yamashina
Jan 15, 1983·The Biochemical Journal·J WildB Winchester
Dec 15, 1994·Structure·H J Hoppe, K B Reid
Feb 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R SastryK N Sastry
Dec 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R A WhiteK N Sastry
Aug 1, 1994·Protein Science : a Publication of the Protein Society·H J Hoppe, K B Reid
Feb 1, 1994·Scandinavian Journal of Immunology·P HoltJ C Jensenius
Apr 1, 1993·Glycobiology·U HolmskovJ C Jensenius
Mar 1, 1996·Scandinavian Journal of Immunology·P StorgaardS E Svehag

❮ Previous
Next ❯

Citations

Mar 26, 2003·The Journal of Biological Chemistry·Shigeyuki TsutsuiYuzuru Suzuki
Nov 22, 2005·Infection and Immunity·Kazue TakahashiR Alan B Ezekowitz
Oct 21, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kazue Takahashi, R Alan B Ezekowitz
Aug 10, 2006·Immunogenetics·Masaru Nonaka, Ayuko Kimura
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miki NakaoTomoki Yano
Apr 23, 2020·Animals : an Open Access Journal From MDPI·Vladimir Zmrhal, Petr Slama
May 1, 1999·The Journal of Biological Chemistry·K OhtaniN Wakamiya
Oct 16, 2004·Genes and Immunity·M V Verga FalzacappaS Crovella
Jun 6, 2012·Journal of Biomedicine & Biotechnology·Wy Ching NgPatrick C Reading
Sep 21, 2001·The Journal of Biological Chemistry·K OhtaniN Wakamiya
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicholas J LynchWilhelm J Schwaeble
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H SekineT Fujita
Oct 12, 2010·Clinical Microbiology Reviews·Sanjay RamPeter A Rice
Mar 5, 2013·Immunogenetics·Rikke M KjærupHelle R Juul-Madsen
Nov 28, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wataru MotomuraNobutaka Wakamiya
Jan 29, 2020·FEBS Letters·Szilvia LukácsiAnna Erdei
Mar 16, 2000·Developmental and Comparative Immunology·S B Laursen, O L Nielsen
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuzuru SuzukiHiroaki Suetake
May 19, 2004·The Journal of Experimental Medicine·Lei ShiR Alan B Ezekowitz
Nov 18, 2005·Molecular Immunology·Astrid HogenkampHenk P Haagsman
Aug 16, 2005·Veterinary Immunology and Immunopathology·Brandon N LillieM Anthony Hayes
Apr 1, 2006·Biochemical and Biophysical Research Communications·Michael J PodolskyKazue Takahashi
Mar 18, 2006·Fish & Shellfish Immunology·Konstantina Nikolakopoulou, Ioannis K Zarkadis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.