Cloning and sequencing of an alpha-glucosidase gene from Aspergillus niger and its expression in A. nidulans

Journal of Biotechnology
A NakamuraT Uozumi

Abstract

We have cloned an extracellular alpha-glucosidase gene from Aspergillus niger with oligonucleotide probes synthesized on the basis of the determined peptide sequences. The nucleotide sequence revealed an open reading frame of 985 amino acids split with three introns, and the deduced amino acid sequence was nearly identical to that of the alpha-glucosidase previously determined. The cloned gene was introduced into Aspergillus nidulans, and its expression in the transformants was shown to be regulated by the carbon sources in the medium, suggesting that a common regulatory expression system is shared by these two species as is the case of other starch-degrading enzymes of Aspergillus species.

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Citations

May 29, 2012·Applied Microbiology and Biotechnology·Ruizhi HanJian Chen
Nov 8, 2012·World Journal of Microbiology & Biotechnology·Xu LiuJian Chen
Feb 22, 2002·European Journal of Biochemistry·Torben P FrandsenBirte Svensson
Jul 18, 2012·BMC Genomics·Mikael R AndersenJens Nielsen
Oct 26, 2005·Bioscience, Biotechnology, and Biochemistry·Fumiaki SatoSeiya Chiba
Dec 27, 2002·The Journal of General and Applied Microbiology·Dong-Gun LeeTakeshi Uozumi
Feb 4, 2016·Applied Microbiology and Biotechnology·Lu WangDepei Wang
Nov 25, 2017·BMC Genomics·Birgit S GrubenRonald P De Vries
Oct 6, 2017·Journal of Agricultural and Food Chemistry·Nannan ZhaoSuiping Zheng

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