PMID: 9540788Apr 16, 1998Paper

Cloning and sequencing of cDNA encoding the LS-12 antifreeze protein in the longhorn sculpin, Myoxocephalus octodecimspinosis

Biochimica Et Biophysica Acta
Z ZhaoR A Laursen

Abstract

cDNA coding for an antifreeze protein (LS-12) in the longhorn sculpin, Myoxocephalus octodecimspinosis, was prepared from liver mRNA using reverse transcriptase-polymerase chain reaction coupled with 3' and 5' RACE procedures. This cDNA contains 609 base pairs, including a 384-bp open reading frame which codes for a 128-residue LS-12 precursor protein. The predicted amino acid sequence of the mature LS-12 corresponds exactly to the amino acid sequence obtained from Edman degradation [G. Deng, D.W. Andrews, R.A. Laursen, FEBS Lett., 402, 1997, pp. 17-20]. The 20 residues preceding mature LS-12 are predicted to be a signal sequence, which is presumably cleaved off before the mature, 108-residue protein is secreted into the circulatory system. This is the first report of a cDNA sequence from M. octodecimspinosis.

References

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Citations

Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·D A WiddickM J Bibb
Oct 22, 2008·Cryobiology·Sherry Y GauthierPeter L Davies
Sep 1, 2005·Journal of Molecular Evolution·Robert P Evans, Garth L Fletcher
Mar 2, 2018·Extremophiles : Life Under Extreme Conditions·Fernanda P CidMilko A Jorquera
Mar 10, 2001·The International Journal of Biochemistry & Cell Biology·J Barrett
Aug 23, 2021·International Journal of Biological Macromolecules·Abirami BaskaranParli V Bhaskar

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