Cloning and sequencing of human lambda immunoglobulin genes by the polymerase chain reaction

European Journal of Immunology
S SongsivilaiN C Hughes-Jones

Abstract

Universal oligonucleotide primers, designed for amplifying and sequencing genes encoding the rearranged human lambda immunoglobulin variable region, were validated by amplification of the lambda light chain genes from four human heterohybridoma cell lines and in the generation of a cDNA library of human V lambda sequences from Epstein-Barr virus-transformed human peripheral blood lymphocytes. This technique allows rapid cloning and sequencing of human immunoglobulin genes, and has potential applications in the rescue of unstable human antibody-producing cell lines and in the production of human monoclonal antibodies.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 25, 1988·Science·M VerhoeyenG Winter
May 15, 1989·Biochemical and Biophysical Research Communications·J W LarrickC A Borrebaeck
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R OrlandiG Winter
Oct 16, 1989·Biochemical and Biophysical Research Communications·S SongsivilaiP J Lachmann
Feb 1, 1986·The Journal of Experimental Medicine·H ChangG F Hollis
Aug 1, 1988·The Journal of Experimental Medicine·S LevyR Levy
Mar 24, 1988·Nature·L RiechmannG Winter
Jan 1, 1988·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·G T StevensonF K Stevenson
Jan 10, 1986·Nucleic Acids Research·R Staden
Dec 13, 1984·Nature·G L BoulianneM J Shulman
Nov 26, 1984·Nucleic Acids Research·Y Tsujimoto, C M Croce
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L MorrisonV T Oi

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Citations

Aug 1, 1994·Molecular Immunology·M F PrummelS M McLachlan
Dec 1, 1994·AIDS Research and Human Retroviruses·E M van der DonkR W van der Heijden
Jan 1, 1993·International Reviews of Immunology·R Billetta, A F Lobuglio
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Satoko OhnoKohzoh Imai
Jul 1, 1993·European Journal of Immunology·S C Williams, G Winter
Dec 1, 1993·Revue française de transfusion et d'hémobiologie : bulletin de la Société nationale de transfusion sanguine·D Goossens

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