PMID: 2125272DOI: 10.1002/eji.1830201220Dec 1, 1990Paper
Cloning and sequencing of human lambda immunoglobulin genes by the polymerase chain reaction
European Journal of Immunology
S SongsivilaiN C Hughes-Jones
Abstract
Universal oligonucleotide primers, designed for amplifying and sequencing genes encoding the rearranged human lambda immunoglobulin variable region, were validated by amplification of the lambda light chain genes from four human heterohybridoma cell lines and in the generation of a cDNA library of human V lambda sequences from Epstein-Barr virus-transformed human peripheral blood lymphocytes. This technique allows rapid cloning and sequencing of human immunoglobulin genes, and has potential applications in the rescue of unstable human antibody-producing cell lines and in the production of human monoclonal antibodies.
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Citations
An anti-lipid A antibody obtained from the human fetal repertoire is encoded by VH6-V lambda 1 genes
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