PMID: 8611161Feb 1, 1996Paper

Cloning and sequencing of rat liver carboxylesterase ES-4 (microsomal palmitoyl-CoA hydrolase)

The Biochemical Journal
M RobbiH Beaufay

Abstract

A cDNA which encodes a carboxylesterase of 561 amino acid residues including a cleavable signal peptide is described. The enzyme expressed in COS cells migrates during PAGE (SDS-, and non-denaturing) as a single prominent band in the region of liver ES-4. It ends in the C-terminal cell-retention signal -HNEL, which, in COS cells overexpressing the enzyme, appears to be slightly less efficient than the signals -HTEL and -HVEL of ES-3 and ES-10 respectively. Glycosylation is not essential for intracellular retention, but leads to a higher activity. As do many carboxylesterases, the enzyme expressed in COS cells hydrolyses omicron-nitrophenyl acetate and alpha-naphthyl acetate. It also hydrolyses acetanilide, although less efficiently than ES-3, and, distinctively, palmitoyl-CoA. In addition to the four canonical Cys residues of the carboxylesterases, it contains a fifth, unpaired Cys336, which apparently is not essential for the catalytic properties. Indeed, treatment with iodoacetamide or substitution of Cys336 by Phe does not markedly alter the activity of the enzyme on the various substrates. The predicted structure of ES-4 is highly homologous to that of two other recently cloned esterases which also end in -HNEL [Yan, Yang, ...Continue Reading

Citations

Sep 17, 2002·European Journal of Biochemistry·Sonal P SanghaniWilliam F Bosron
Dec 9, 2003·The Journal of Biological Chemistry·Gregory B TiltonJohn Browse
Jul 13, 2007·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S YamaoriK Watanabe
Feb 29, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Masakiyo Hosokawa
Sep 23, 2011·The Journal of Biological Chemistry·Saj ParathathEdward A Fisher
May 23, 1998·Annual Review of Pharmacology and Toxicology·T Satoh, M Hosokawa
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·T J WallaceW McLean Grogan
Sep 25, 1999·Protein Expression and Purification·D F ScottK D Lanclos
Jan 5, 2002·Progress in Lipid Research·Mary C Hunt, Stefan E H Alexson
Jul 9, 1999·Biochimica Et Biophysica Acta·M A DiczfalusyS E Alexson

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