PMID: 11934525Apr 6, 2002Paper

Cloning and sequencing of the 3'-region of the canine parathyroid hormone-related protein gene and analysis of alternate mRNA splicing in two canine carcinomas

Domestic Animal Endocrinology
A GröneT J Rosol

Abstract

A canine genomic library in Lambda FIX II vector was screened with a 281-base pair canine PTHrP cDNA to the prepro- and coding regions. Two genomic clones were isolated and mapped to the 3'-end of the PTHrP gene by polymerase chain reaction (PCR) amplification of exons in this region. One clone (3.5 kb) was amplified by PCR, partially sequenced, and compared to the human PTHrP gene. Regions were identified with a high degree of homology to exons 6, 7, and 8 of the human PTHrP gene. A polyadenylation site was present 3' to the exon 8-like region. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that exon 7 of the PTHrP gene was transcribed in two canine carcinomas (SCC 2/88 cells and CAC-8 tumor line) which produce PTHrP. This confirmed that the 3'-region of the canine PTHrP gene is alternately spliced with splicing of exon 6 to exons 7 or 9. Transcription of exon 8 was not demonstrated by RT-PCR and suggests that the exon 8-like region of the dog PTHrP gene is not utilized. The exon 8-like region contained an early stop codon that was not present in exon 8 of the human PTHrP gene.

References

Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D T SchermerG J Strewler
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M ManginA E Broadus
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Citations

Aug 5, 2004·Journal of Molecular Endocrinology·R S SellersT J Rosol

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