Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

Gene
M K StevensE J Hansen

Abstract

The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

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Citations

Feb 21, 2002·Infection and Immunity·Cliffton T H BongStanley M Spinola
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·F C FangT Laessig
Aug 21, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Shahin NegariB Craig Lee
Mar 10, 2015·Journal of Advanced Research·M AbdelsalamS-C Chen

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