Cloning and soluble expression of mature α-luffin from Luffa cylindrica in E. coli using SUMO fusion protein

Turkish Journal of Biology = Türk Biyoloji Dergisi
Shaghayegh NamvarFatemeh Davami

Abstract

α-Lufin, found in Luaf cylindrica seeds, is a type I ribosome inactivating proteins. Cytotoxic effects make it an appropriate candidate for the construction of immunotoxins and conjugates. Because of limited natural resources, recombinant technology is the best approach to achieve large-scale production of plant-based proteins. In the present study, α-lufin protein was expressed in E. coli and the effects of different temperature conditions, SUMO fusion tag, and cultivation strategies on total expression and solubility were investigated. Protein expression was evaluated at different intervals (0, 4, 6, 8, 24 h) postinduction. Our results showed that EnBase had higher eficiency than LB, and maximum solubility and total protein expression were achieved 24 h after induction at 30 °C and 25 °C, respectively. It was shown that SUMO tag is an effective strategy to improve protein solubility.

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Citations

Jun 4, 2020·Saudi Journal of Biological Sciences·Masoumeh Rezaei-MoshaeiMajid Golkar

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